|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5wtx' size='340' side='right'caption='[[5wtx]], [[Resolution|resolution]] 3.07Å' scene=''> | | <StructureSection load='5wtx' size='340' side='right'caption='[[5wtx]], [[Resolution|resolution]] 3.07Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wtx]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Baker's_yeast Baker's yeast]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTX OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WTX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wtx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WTX FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wty|5wty]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.074Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NOP9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=559292 Baker's yeast])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtx OCA], [https://pdbe.org/5wtx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wtx RCSB], [https://www.ebi.ac.uk/pdbsum/5wtx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtx ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wtx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wtx OCA], [http://pdbe.org/5wtx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wtx RCSB], [http://www.ebi.ac.uk/pdbsum/5wtx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wtx ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST]] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref> | + | [https://www.uniprot.org/uniprot/NOP9_YEAST NOP9_YEAST] RNA-binding nucleolar protein required for pre-rRNA processing. Component of the 90S pre-ribosome involved in production of 18S rRNA and assembly of small ribosomal subunit. Component of the pre-40S ribosome required for release from the nucleolus.<ref>PMID:16544271</ref> <ref>PMID:17956976</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | The assembly of eukaryotic ribosomes requires numerous factors that transiently associate with evolving pre-ribosomal particles. The Pumilio repeat-containing protein Nop9 briefly associates with the 90S pre-ribosome during its co-transcriptional assembly. Here, we show that Nop9 specifically binds an 11-nucleotide sequence of 18S rRNA that forms the 3' side of the central pseudoknot and helix 28 in the mature subunit. Crystal structures of Nop9 in the free and RNA-bound states reveal a new type of Pumilio repeat protein with a distinct structure, target sequence and RNA-binding mode. Nop9 contains 10 Pumilio repeats arranged into a U-shaped scaffold. The target RNA is recognized by two stretches of repeats in a bipartite manner, and three central bases are unrecognized as a result of the degeneracy of repeats 6 and 7. Our data suggest that Nop9 regulates the folding of 18S rRNA at early assembly stages of 90S.
| + | |
| - | | + | |
| - | Nop9 binds the central pseudoknot region of 18S rRNA.,Wang B, Ye K Nucleic Acids Res. 2017 Jan 3. pii: gkw1323. doi: 10.1093/nar/gkw1323. PMID:28053123<ref>PMID:28053123</ref>
| + | |
| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5wtx" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Baker's yeast]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Wang, B]] | + | [[Category: Saccharomyces cerevisiae S288C]] |
| - | [[Category: Ye, K]] | + | [[Category: Wang B]] |
| - | [[Category: Pumilio repeat-containing protein]] | + | [[Category: Ye K]] |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Rna-binding protein]]
| + | |
