|
|
| Line 3: |
Line 3: |
| | <StructureSection load='5wyg' size='340' side='right'caption='[[5wyg]], [[Resolution|resolution]] 2.36Å' scene=''> | | <StructureSection load='5wyg' size='340' side='right'caption='[[5wyg]], [[Resolution|resolution]] 2.36Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wyg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Myctu Myctu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5WYG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wyg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Rv Mycobacterium tuberculosis H37Rv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WYG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WYG FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mazF7, Rv2063A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83332 MYCTU])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.356Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5wyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wyg OCA], [http://pdbe.org/5wyg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wyg RCSB], [http://www.ebi.ac.uk/pdbsum/5wyg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wyg ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wyg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wyg OCA], [https://pdbe.org/5wyg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wyg RCSB], [https://www.ebi.ac.uk/pdbsum/5wyg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wyg ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MAZF7_MYCTU MAZF7_MYCTU]] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an endoribonuclease (By similarity).[UniProtKB:P9WIH9]<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> | + | [https://www.uniprot.org/uniprot/MAZF7_MYCTU MAZF7_MYCTU] Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an endoribonuclease (By similarity).[UniProtKB:P9WIH9]<ref>PMID:19016878</ref> <ref>PMID:20011113</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myctu]] | + | [[Category: Mycobacterium tuberculosis H37Rv]] |
| - | [[Category: Chen, R]] | + | [[Category: Chen R]] |
| - | [[Category: Tu, J]] | + | [[Category: Tu J]] |
| - | [[Category: Xie, W]] | + | [[Category: Xie W]] |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Mazf]]
| + | |
| - | [[Category: Mycobacterium tuberculosis]]
| + | |
| - | [[Category: Toxin-antitoxin system]]
| + | |
| Structural highlights
Function
MAZF7_MYCTU Toxic component of a type II toxin-antitoxin (TA) module. Upon expression in E.coli and M.smegmatis inhibits cell growth and colony formation. Its toxic effect is neutralized by coexpression with cognate antitoxin MazE7 (PubMed:19016878, PubMed:20011113). Probably an endoribonuclease (By similarity).[UniProtKB:P9WIH9][1] [2]
Publication Abstract from PubMed
Tuberculosis (TB) is a severe disease caused by Mycobacterium tuberculosis (M. tb) and the well-characterized M. tb MazE/F proteins play important roles in stress adaptation. Recently, the MazF-mt9 toxin has been found to display endonuclease activities towards tRNAs but the mechanism is unknown. We hereby present the crystal structure of apo-MazF-mt9. The enzyme recognizes tRNALys with a central UUU motif within the anticodon loop, but is insensitive to the sequence context outside of the loop. Based on our crystallographic and biochemical studies, we identified key residues for catalysis and proposed the potential tRNA-binding site.
Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis.,Chen R, Tu J, Liu Z, Meng F, Ma P, Ding Z, Yang C, Chen L, Deng X, Xie W Biochem Biophys Res Commun. 2017 May 6;486(3):804-810. doi:, 10.1016/j.bbrc.2017.03.132. Epub 2017 Mar 25. PMID:28351618[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gupta A. Killing activity and rescue function of genome-wide toxin-antitoxin loci of Mycobacterium tuberculosis. FEMS Microbiol Lett. 2009 Jan;290(1):45-53. doi:, 10.1111/j.1574-6968.2008.01400.x. Epub 2008 Nov 10. PMID:19016878 doi:http://dx.doi.org/10.1111/j.1574-6968.2008.01400.x
- ↑ Ramage HR, Connolly LE, Cox JS. Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genet. 2009 Dec;5(12):e1000767. doi: 10.1371/journal.pgen.1000767. Epub 2009, Dec 11. PMID:20011113 doi:http://dx.doi.org/10.1371/journal.pgen.1000767
- ↑ Chen R, Tu J, Liu Z, Meng F, Ma P, Ding Z, Yang C, Chen L, Deng X, Xie W. Structure of the MazF-mt9 toxin, a tRNA-specific endonuclease from Mycobacterium tuberculosis. Biochem Biophys Res Commun. 2017 May 6;486(3):804-810. doi:, 10.1016/j.bbrc.2017.03.132. Epub 2017 Mar 25. PMID:28351618 doi:http://dx.doi.org/10.1016/j.bbrc.2017.03.132
|
