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| | <StructureSection load='6tg6' size='340' side='right'caption='[[6tg6]], [[Resolution|resolution]] 1.30Å' scene=''> | | <StructureSection load='6tg6' size='340' side='right'caption='[[6tg6]], [[Resolution|resolution]] 1.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6tg6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TG6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TG6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tg6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_stearothermophilus Geobacillus stearothermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TG6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TG6 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnmV_1, rnmV, AVP43_02013 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3Å</td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_M5 Ribonuclease M5], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.8 3.1.26.8] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tg6 OCA], [https://pdbe.org/6tg6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tg6 RCSB], [https://www.ebi.ac.uk/pdbsum/6tg6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tg6 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tg6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tg6 OCA], [http://pdbe.org/6tg6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tg6 RCSB], [http://www.ebi.ac.uk/pdbsum/6tg6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tg6 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A0A250DVN1_GEOSE A0A250DVN1_GEOSE] Required for correct processing of both the 5' and 3' ends of 5S rRNA precursor. Cleaves both sides of a double-stranded region yielding mature 5S rRNA in one step.[HAMAP-Rule:MF_01469] |
| - | The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 A resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing.
| + | |
| | | | |
| - | Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.,Oerum S, Dendooven T, Catala M, Gilet L, Degut C, Trinquier A, Bourguet M, Barraud P, Cianferani S, Luisi BF, Condon C, Tisne C Mol Cell. 2020 Sep 23. pii: S1097-2765(20)30616-X. doi:, 10.1016/j.molcel.2020.09.008. PMID:32991829<ref>PMID:32991829</ref>
| + | ==See Also== |
| - | | + | *[[Ribonuclease 3D structures|Ribonuclease 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 6tg6" style="background-color:#fffaf0;"></div>
| + | |
| - | == References == | + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 12980]] | + | [[Category: Geobacillus stearothermophilus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Ribonuclease M5]]
| + | [[Category: Catala M]] |
| - | [[Category: Catala, M]] | + | [[Category: Oerum S]] |
| - | [[Category: Oerum, S]] | + | [[Category: Tisne C]] |
| - | [[Category: Tisne, C]] | + | |
| - | [[Category: 5s rrna]]
| + | |
| - | [[Category: Catalytic domain]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Pre-5s rrna]]
| + | |
| - | [[Category: Precursor rrna]]
| + | |
| - | [[Category: Ribonuclease]]
| + | |
| - | [[Category: Ribosomal rna]]
| + | |
| - | [[Category: Rnase]]
| + | |
| - | [[Category: Rnase m5]]
| + | |
| - | [[Category: Rrna]]
| + | |
| - | [[Category: Toprim domain]]
| + | |
