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5xe8

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Crystal Structure of the Peptidase Domain of Streptococcus mutans ComA

Structural highlights

5xe8 is a 2 chain structure with sequence from Streptococcus mutans UA159. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 3.1Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q8DW05_STRMU

Publication Abstract from PubMed

The main components of the quorum-sensing system are expected to be favorable targets for drug development to combat various chronic infectious diseases. ComA of Streptococcus is an ATP-binding cassette transporter containing a peptidase domain (PEP), which is essential for the quorum-sensing signal production. Using high-throughput screening, we found a potent small molecule that suppressed the S. mutans quorum-sensing pathway through inhibition of PEP activity. The compound effectively attenuated the biofilm formation and competence development of S. mutans without inhibiting cell growth. The kinetic and structural studies with this molecule and a related compound unexpectedly revealed an allosteric site of PEP. This relatively hydrophobic site is thought to undergo large structural changes during the catalytic process. These compounds inhibit PEP activity by binding to and suppressing the structural changes of this site. These results showed that PEP is a good target for inhibitors of the Streptococcus quorum-sensing system.

High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway.,Ishii S, Fukui K, Yokoshima S, Kumagai K, Beniyama Y, Kodama T, Fukuyama T, Okabe T, Nagano T, Kojima H, Yano T Sci Rep. 2017 Jun 22;7(1):4029. doi: 10.1038/s41598-017-03567-2. PMID:28642545^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Ishii S, Fukui K, Yokoshima S, Kumagai K, Beniyama Y, Kodama T, Fukuyama T, Okabe T, Nagano T, Kojima H, Yano T. High-throughput Screening of Small Molecule Inhibitors of the Streptococcus Quorum-sensing Signal Pathway. Sci Rep. 2017 Jun 22;7(1):4029. doi: 10.1038/s41598-017-03567-2. PMID:28642545 doi:http://dx.doi.org/10.1038/s41598-017-03567-2

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xe8"

@@ Line 3: / Line 3: @@
 <StructureSection load='5xe8' size='340' side='right'caption='[[5xe8]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xe8]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XE8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XE8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xe8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_mutans_UA159 Streptococcus mutans UA159]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XE8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XE8 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xe9|5xe9]]</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xe8 OCA], [http://pdbe.org/5xe8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xe8 RCSB], [http://www.ebi.ac.uk/pdbsum/5xe8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xe8 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xe8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xe8 OCA], [https://pdbe.org/5xe8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xe8 RCSB], [https://www.ebi.ac.uk/pdbsum/5xe8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xe8 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q8DW05_STRMU Q8DW05_STRMU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Beniyama, Y]]
+[[Category: Streptococcus mutans UA159]]
-[[Category: Fukui, K]]
+[[Category: Beniyama Y]]
-[[Category: Fukuyama, T]]
+[[Category: Fukui K]]
-[[Category: Ishii, S]]
+[[Category: Fukuyama T]]
-[[Category: Kodama, T]]
+[[Category: Ishii S]]
-[[Category: Kojima, H]]
+[[Category: Kodama T]]
-[[Category: Kumagai, K]]
+[[Category: Kojima H]]
-[[Category: Nagano, T]]
+[[Category: Kumagai K]]
-[[Category: Okabe, T]]
+[[Category: Nagano T]]
-[[Category: Yano, T]]
+[[Category: Okabe T]]
-[[Category: Yokoshima, S]]
+[[Category: Yano T]]
-[[Category: Hydrolase-hydrolase inhibitor complex]]
+[[Category: Yokoshima S]]
-[[Category: Peptidase]]
-[[Category: Quorum-sensing]]

5xe8

From Proteopedia

Current revision

Crystal Structure of the Peptidase Domain of Streptococcus mutans ComA

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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