|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6jmt' size='340' side='right'caption='[[6jmt]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6jmt' size='340' side='right'caption='[[6jmt]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6jmt]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JMT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6JMT FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jmt]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JMT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JMT FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Git2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6jmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jmt OCA], [http://pdbe.org/6jmt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jmt RCSB], [http://www.ebi.ac.uk/pdbsum/6jmt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jmt ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jmt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jmt OCA], [https://pdbe.org/6jmt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jmt RCSB], [https://www.ebi.ac.uk/pdbsum/6jmt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jmt ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/GIT2_MOUSE GIT2_MOUSE] GTPase-activating protein for ADP ribosylation factor family members, including ARF1.[UniProtKB:Q14161] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 24: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Lin, L]] | + | [[Category: Lin L]] |
| - | [[Category: Xia, Y]] | + | [[Category: Xia Y]] |
| - | [[Category: Zhang, M]] | + | [[Category: Zhang M]] |
| - | [[Category: Zhang, R]] | + | [[Category: Zhang R]] |
| - | [[Category: Zhu, J]] | + | [[Category: Zhu J]] |
| - | [[Category: Cell adhesion]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Git2]]
| + | |
| - | [[Category: Pix]]
| + | |
| Structural highlights
Function
GIT2_MOUSE GTPase-activating protein for ADP ribosylation factor family members, including ARF1.[UniProtKB:Q14161]
Publication Abstract from PubMed
Enzymes or enzyme complexes can be concentrated in different cellular loci to modulate distinct functional processes in response to specific signals. How cells condense and compartmentalize enzyme complexes for spatiotemporally distinct cellular events is not well understood. Here we discover that specific and tight association of GIT1 and beta-Pix, a pair of GTPase regulatory enzymes, leads to phase separation of the complex without additional scaffolding molecules. GIT1/beta-Pix condensates are modular in nature and can be positioned at distinct cellular compartments, such as neuronal synapses, focal adhesions, and cell-cell junctions, by upstream adaptors. Guided by the structure of the GIT/PIX complex, we specifically probed the role of phase separation of the enzyme complex in cell migration and synapse formation. Our study suggests that formation of modular enzyme complex condensates via phase separation can dynamically concentrate limited quantities of enzymes to distinct cellular compartments for specific and optimal signaling.
GIT/PIX Condensates Are Modular and Ideal for Distinct Compartmentalized Cell Signaling.,Zhu J, Zhou Q, Xia Y, Lin L, Li J, Peng M, Zhang R, Zhang M Mol Cell. 2020 Sep 3;79(5):782-796.e6. doi: 10.1016/j.molcel.2020.07.004. Epub, 2020 Aug 10. PMID:32780989[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zhu J, Zhou Q, Xia Y, Lin L, Li J, Peng M, Zhang R, Zhang M. GIT/PIX Condensates Are Modular and Ideal for Distinct Compartmentalized Cell Signaling. Mol Cell. 2020 Sep 3;79(5):782-796.e6. doi: 10.1016/j.molcel.2020.07.004. Epub, 2020 Aug 10. PMID:32780989 doi:http://dx.doi.org/10.1016/j.molcel.2020.07.004
|
