6zfc
From Proteopedia
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==Fucose-binding lectin from Burkholderia ambifaria (BamBL) in complex with a fucosyl derivative== | ==Fucose-binding lectin from Burkholderia ambifaria (BamBL) in complex with a fucosyl derivative== | ||
| - | <StructureSection load='6zfc' size='340' side='right'caption='[[6zfc]]' scene=''> | + | <StructureSection load='6zfc' size='340' side='right'caption='[[6zfc]], [[Resolution|resolution]] 1.65Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZFC OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6zfc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_ambifaria_AMMD Burkholderia ambifaria AMMD]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZFC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZFC FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=QJB:2-[(2~{S},3~{S},4~{R},4~{a}~{S},10~{a}~{S})-2-methyl-3,4-bis(oxidanyl)-3,4,4~{a},10~{a}-tetrahydro-2~{H}-pyrano[2,3-b][1,4]benzoxathiin-7-yl]-~{N}-(3-oxidanylpropyl)ethanamide'>QJB</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zfc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zfc OCA], [https://pdbe.org/6zfc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zfc RCSB], [https://www.ebi.ac.uk/pdbsum/6zfc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zfc ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q0B4G1_BURCM Q0B4G1_BURCM] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Two orthogonal, metal free click reactions, enabled to glycosylate ubiquitin and its mutant A28C forming two protein scaffolds with high affinity for BambL, a lectin from the human pathogen Burkholderia ambifaria. A new fucoside analogue, with high affinity with BambL, firstly synthetized and co-crystallized with the protein target, provided the insights for sugar determinants grafting onto ubiquitin. Three ubiquitin-based glycosides were thus assembled. Fuc-Ub, presented several copies of the fucoside analogue, with proper geometry for multivalent effect; Rha-A28C, displayed one thio-rhamnose, known for its ability to tuning the immunological response; finally, Fuc-Rha-A28C, included both multiple fucoside analogs and the rhamnose residue. Fuc-Ub and Fuc-Rha-A28C ligands proved high affinity for BambL and unprecedented immune modulatory properties towards macrophages activation. | ||
| + | |||
| + | Fucosylated ubiquitin and orthogonally glycosylated mutant A28C: conceptually new ligands for Burkholderia ambifaria lectin (BambL).,Kuhaudomlarp S, Cerofolini L, Santarsia S, Gillon E, Fallarini S, Lombardi G, Denis M, Giuntini S, Valori C, Fragai M, Imberty A, Dondoni A, Nativi C Chem Sci. 2020 Oct 21;11(47):12662-12670. doi: 10.1039/d0sc03741a. PMID:34094460<ref>PMID:34094460</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zfc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Burkholderia ambifaria AMMD]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cerofolini L]] | [[Category: Cerofolini L]] | ||
Current revision
Fucose-binding lectin from Burkholderia ambifaria (BamBL) in complex with a fucosyl derivative
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