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4cvd

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Crystal structure of the central repeat of cell wall binding module of Cpl7

Structural highlights

4cvd is a 1 chain structure with sequence from Streptococcus phage CP-7. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.666Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

LYS_BPCP7 Responsible for the separation of the host daughter cells at the end of cell division and participates in the liberation of progeny bacteriophage into the medium. Degrades cell walls containing either choline or ethanolamine.

Publication Abstract from PubMed

Endolysins, the cell wall lytic enzymes encoded by bacteriophages to release the phage progeny, are among the top alternatives to fight against multiresistant pathogenic bacteria; one of the current biggest challenges to global health. Their narrow range of susceptible bacteria relies, primarily, on targeting specific cell-wall receptors through specialized modules. The cell wall-binding domain of Cpl-7 endolysin, made of three CW_7 repeats, accounts for its extended-range of substrates. Using as model system the cell wall-binding domain of Cpl-7, here we describe the molecular basis for the bacterial cell wall recognition by the CW_7 motif, which is widely represented in sequences of cell wall hydrolases. We report the crystal and solution structure of the full-length domain, identify N-acetyl-D-glucosaminyl-(beta1,4)-N-acetylmuramyl-L-alanyl-D-isoglutamine (GMDP) as the peptidoglycan (PG) target recognized by the CW_7 motifs, and characterize feasible GMDP-CW_7 contacts. Our data suggest that Cpl-7 cell wall-binding domain might simultaneously bind to three PG chains, and also highlight the potential use of CW_7-containing lysins as novel anti-infectives.

Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan.,Bustamante N, Iglesias-Bexiga M, Bernardo-Garcia N, Silva-Martin N, Garcia G, Campanero-Rhodes MA, Garcia E, Uson I, Buey RM, Garcia P, Hermoso JA, Bruix M, Menendez M Sci Rep. 2017 Nov 28;7(1):16494. doi: 10.1038/s41598-017-16392-4. PMID:29184076^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bustamante N, Iglesias-Bexiga M, Bernardo-Garcia N, Silva-Martin N, Garcia G, Campanero-Rhodes MA, Garcia E, Uson I, Buey RM, Garcia P, Hermoso JA, Bruix M, Menendez M. Deciphering how Cpl-7 cell wall-binding repeats recognize the bacterial peptidoglycan. Sci Rep. 2017 Nov 28;7(1):16494. doi: 10.1038/s41598-017-16392-4. PMID:29184076 doi:http://dx.doi.org/10.1038/s41598-017-16392-4

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4cvd"

@@ Line 3: / Line 3: @@
 <StructureSection load='4cvd' size='340' side='right'caption='[[4cvd]], [[Resolution|resolution]] 1.67&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4cvd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpcp7 Bpcp7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CVD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=4CVD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4cvd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_CP-7 Streptococcus phage CP-7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CVD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CVD FirstGlance]. <br>
-</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.666&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=4cvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cvd OCA], [http://pdbe.org/4cvd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4cvd RCSB], [http://www.ebi.ac.uk/pdbsum/4cvd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4cvd ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4cvd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4cvd OCA], [https://pdbe.org/4cvd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4cvd RCSB], [https://www.ebi.ac.uk/pdbsum/4cvd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4cvd ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/LYS_BPCP7 LYS_BPCP7]] Responsible for the separation of the host daughter cells at the end of cell division and participates in the liberation of progeny bacteriophage into the medium. Degrades cell walls containing either choline or ethanolamine.
+[https://www.uniprot.org/uniprot/LYS_BPCP7 LYS_BPCP7] Responsible for the separation of the host daughter cells at the end of cell division and participates in the liberation of progeny bacteriophage into the medium. Degrades cell walls containing either choline or ethanolamine.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 20: / Line 20: @@
 ==See Also==
-*[[Lysin|Lysin]]
+*[[Lysin 3D structures|Lysin 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
-[[Category: Bpcp7]]
 [[Category: Large Structures]]
-[[Category: Lysozyme]]
+[[Category: Streptococcus phage CP-7]]
-[[Category: Hermoso, J A]]
+[[Category: Hermoso JA]]
-[[Category: Rodriguez, D D]]
+[[Category: Rodriguez DD]]
-[[Category: Silva-Martin, N]]
+[[Category: Silva-Martin N]]
-[[Category: Uson, I]]
+[[Category: Uson I]]
-[[Category: Hydrolase]]
-[[Category: Streptococcus pneumoniae]]

4cvd

From Proteopedia

Current revision

Crystal structure of the central repeat of cell wall binding module of Cpl7

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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