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1xpf

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(New page: 200px<br /> <applet load="1xpf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xpf, resolution 2.30&Aring;" /> '''HIV-1 subtype A gen...)
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[[Image:1xpf.gif|left|200px]]<br />
 
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<applet load="1xpf" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1xpf, resolution 2.30&Aring;" />
 
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'''HIV-1 subtype A genomic RNA Dimerization Initiation Site'''<br />
 
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==Overview==
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==HIV-1 subtype A genomic RNA Dimerization Initiation Site==
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All retroviruses encapsidate their genome as a dimer of homologous, single-stranded RNAs. The dimerization initiation site (DIS) of human, immunodeficiency virus type 1 (HIV-1) is located in the 5'-untranslated, region of the viral genome and consists of a hairpin with a 6 nt, self-complementary loop sequence. Genomic RNA dimerization, a crucial step, for virion infectivity, is promoted by the formation of a loop-loop, complex (or kissing complex) between two DIS hairpins. Crystal structures, for the subtypes A, B and F of the HIV-1 DIS kissing complex have now been, solved at 2.3 A, 1.9 A and 1.6 A, respectively. They revealed a, polymorphism of bulged-out residues showing clearly that their, conformation is not a mere consequence of crystal packing. They also, provide more insights into ion binding, hydration, and RNA conformation, and flexibility. In particular, we observed the binding of spermine to the, loop-loop helix, which displaced a magnesium cation important for subtype, A DIS dimerization. The excellent agreement between X-ray structures and, the results of chemical probing and interference data on larger viral RNA, fragments shows that the crystal structures are relevant for the DIS, kissing complex present in solution and in viral particles. Accordingly, these structures will be helpful for designing new drugs derived from, aminoglycoside antibiotics and targeted against the RNA dimerization step, of the viral life-cycle.
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<StructureSection load='1xpf' size='340' side='right'caption='[[1xpf]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1xpf]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XPF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XPF FirstGlance]. <br>
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1XPF is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with SO4, MG, NA and SPM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1XPF OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=SPM:SPERMINE'>SPM</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xpf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xpf OCA], [https://pdbe.org/1xpf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xpf RCSB], [https://www.ebi.ac.uk/pdbsum/1xpf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xpf ProSAT]</span></td></tr>
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Polymorphism of bulged-out residues in HIV-1 RNA DIS kissing complex and structure comparison with solution studies., Ennifar E, Dumas P, J Mol Biol. 2006 Feb 24;356(3):771-82. Epub 2005 Dec 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403527 16403527]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Dumas, P.]]
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</StructureSection>
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[[Category: Ennifar, E.]]
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[[Category: Large Structures]]
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[[Category: MG]]
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[[Category: Dumas P]]
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[[Category: NA]]
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[[Category: Ennifar E]]
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[[Category: SO4]]
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[[Category: SPM]]
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[[Category: hiv-1]]
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[[Category: loop-loop complex]]
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[[Category: rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:35:57 2007''
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Current revision

HIV-1 subtype A genomic RNA Dimerization Initiation Site

PDB ID 1xpf

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