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| | <StructureSection load='6h5b' size='340' side='right'caption='[[6h5b]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='6h5b' size='340' side='right'caption='[[6h5b]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6h5b]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25232 Atcc 25232] and [http://en.wikipedia.org/wiki/Myxxd Myxxd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5B OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6H5B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6h5b]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Myxococcus_xanthus Myxococcus xanthus] and [https://en.wikipedia.org/wiki/Myxococcus_xanthus_DK_1622 Myxococcus xanthus DK 1622]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6H5B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6H5B FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mglA, MXAN_1925 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=246197 MYXXD]), mglB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=34 ATCC 25232])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GSP:5-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE'>GSP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6h5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5b OCA], [http://pdbe.org/6h5b PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6h5b RCSB], [http://www.ebi.ac.uk/pdbsum/6h5b PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5b ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6h5b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6h5b OCA], [https://pdbe.org/6h5b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6h5b RCSB], [https://www.ebi.ac.uk/pdbsum/6h5b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6h5b ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MGLA_MYXXD MGLA_MYXXD]] Required for multicellular development and for both mechanisms of gliding: social (S) and adventurous (A) motility. Acts as an intracellular switch to coordinate A and S motilities. Controls the direction of gliding and gliding speed.<ref>PMID:10198026</ref> <ref>PMID:1938957</ref> | + | [https://www.uniprot.org/uniprot/MGLA_MYXXD MGLA_MYXXD] Required for multicellular development and for both mechanisms of gliding: social (S) and adventurous (A) motility. Acts as an intracellular switch to coordinate A and S motilities. Controls the direction of gliding and gliding speed.<ref>PMID:10198026</ref> <ref>PMID:1938957</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 25232]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Myxxd]] | + | [[Category: Myxococcus xanthus]] |
| - | [[Category: Cherfils, J]] | + | [[Category: Myxococcus xanthus DK 1622]] |
| - | [[Category: Galicia, C]] | + | [[Category: Cherfils J]] |
| - | [[Category: Cytosolic protein]] | + | [[Category: Galicia C]] |
| - | [[Category: Gtpase and gap complex]]
| + | |
| Structural highlights
6h5b is a 3 chain structure with sequence from Myxococcus xanthus and Myxococcus xanthus DK 1622. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.8Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
MGLA_MYXXD Required for multicellular development and for both mechanisms of gliding: social (S) and adventurous (A) motility. Acts as an intracellular switch to coordinate A and S motilities. Controls the direction of gliding and gliding speed.[1] [2]
Publication Abstract from PubMed
In Myxococcus xanthus, directed movement is controlled by pole-to-pole oscillations of the small GTPase MglA and its GAP MglB. Direction reversals require that MglA is inactivated by MglB, yet paradoxically MglA and MglB are located at opposite poles at reversal initiation. Here we report the complete MglA/MglB structural cycle combined to GAP kinetics and in vivo motility assays, which uncovers that MglA is a three-state GTPase and suggests a molecular mechanism for concerted MglA/MglB relocalizations. We show that MglA has an atypical GTP-bound state (MglA-GTP*) that is refractory to MglB and is re-sensitized by a feedback mechanism operated by MglA-GDP. By identifying and mutating the pole-binding region of MglB, we then provide evidence that the MglA-GTP* state exists in vivo. These data support a model in which MglA-GDP acts as a soluble messenger to convert polar MglA-GTP* into a diffusible MglA-GTP species that re-localizes to the opposite pole during reversals.
MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus.,Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J Nat Commun. 2019 Nov 22;10(1):5300. doi: 10.1038/s41467-019-13274-3. PMID:31757955[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Spormann AM, Kaiser D. Gliding mutants of Myxococcus xanthus with high reversal frequencies and small displacements. J Bacteriol. 1999 Apr;181(8):2593-601. PMID:10198026
- ↑ Hartzell P, Kaiser D. Function of MglA, a 22-kilodalton protein essential for gliding in Myxococcus xanthus. J Bacteriol. 1991 Dec;173(23):7615-24. PMID:1938957
- ↑ Galicia C, Lhospice S, Varela PF, Trapani S, Zhang W, Navaza J, Herrou J, Mignot T, Cherfils J. MglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus. Nat Commun. 2019 Nov 22;10(1):5300. doi: 10.1038/s41467-019-13274-3. PMID:31757955 doi:http://dx.doi.org/10.1038/s41467-019-13274-3
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