6cle

<table><tr><td colspan='2'>[[6cle]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6CLE FirstGlance]. <br>

</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>

<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6cle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cle OCA], [http://pdbe.org/6cle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cle RCSB], [http://www.ebi.ac.uk/pdbsum/6cle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cle ProSAT]</span></td></tr>

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== Publication Abstract from PubMed ==

Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data.

Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.,Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T Structure. 2018 May 1;26(5):759-766.e4. doi: 10.1016/j.str.2018.03.021. Epub 2018, Apr 26. PMID:29706530<ref>PMID:29706530</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>

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<div class="pdbe-citations 6cle" style="background-color:#fffaf0;"></div>

== References ==

<references/>

[[Category: Gallagher-Jones, M]]

[[Category: Glynn, C]]

[[Category: Gonen, T]]

[[Category: Hattne, J]]

[[Category: Martynowycz, M W]]

[[Category: Rodriguez, J A]]

[[Category: Shi, D]]

[[Category: Zee, C T]]

[[Category: Zinc binding]]