6d0h

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Current revision (14:31, 13 March 2024) (edit) (undo)
 
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<StructureSection load='6d0h' size='340' side='right'caption='[[6d0h]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
<StructureSection load='6d0h' size='340' side='right'caption='[[6d0h]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6d0h]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Sphyb Sphyb]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D0H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6D0H FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6d0h]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Sphingobium_sp._YBL2 Sphingobium sp. YBL2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D0H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D0H FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.5&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TZ53_17660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=484429 SPHYB]), TZ53_17665 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=484429 SPHYB])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6d0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d0h OCA], [http://pdbe.org/6d0h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d0h RCSB], [http://www.ebi.ac.uk/pdbsum/6d0h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d0h ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d0h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d0h OCA], [https://pdbe.org/6d0h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d0h RCSB], [https://www.ebi.ac.uk/pdbsum/6d0h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d0h ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/PART_SPHYB PART_SPHYB] Toxic component of a type II toxin-antitoxin (TA) system. Expression in E.coli inhibits cell growth; bacteriostasis is neutralized by expression of cognate antitoxin ParS. ADP-ribosylates E.coli ribose-phosphate pyrophosphokinase (RPPK, prs) using NAD(+) in vitro; ADP-ribosylates RPPK on 'Lys-182' and 'Ser-202'. Cannot use NADP(+). Also auto-ADP-ribosylates in vitro; in the presence of RPPK auto-ADP-ribosylation decreases.<ref>PMID:30598453</ref>
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Toxin-antitoxin (TA) systems interfere with essential cellular processes and are implicated in bacterial lifestyle adaptations such as persistence and the biofilm formation. Here, we present structural, biochemical, and functional data on an uncharacterized TA system, the COG5654-COG5642 pair. Bioinformatic analysis showed that this TA pair is found in 2,942 of the 16,286 distinct bacterial species in the RefSeq database. We solved a structure of the toxin bound to a fragment of the antitoxin to 1.50 A. This structure suggested that the toxin is a mono-ADP-ribosyltransferase (mART). The toxin specifically modifies phosphoribosyl pyrophosphate synthetase (Prs), an essential enzyme in nucleotide biosynthesis conserved in all organisms. We propose renaming the toxin ParT for Prs ADP-ribosylating toxin and ParS for the cognate antitoxin. ParT is a unique example of an intracellular protein mART in bacteria and is the smallest known mART. This work demonstrates that TA systems can induce bacteriostasis through interference with nucleotide biosynthesis.
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ParST is a widespread toxin-antitoxin module that targets nucleotide metabolism.,Piscotta FJ, Jeffrey PD, Link AJ Proc Natl Acad Sci U S A. 2018 Dec 31. pii: 1814633116. doi:, 10.1073/pnas.1814633116. PMID:30598453<ref>PMID:30598453</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6d0h" style="background-color:#fffaf0;"></div>
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Sphyb]]
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[[Category: Sphingobium sp. YBL2]]
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[[Category: Jeffrey, P D]]
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[[Category: Jeffrey PD]]
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[[Category: Link, A J]]
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[[Category: Link AJ]]
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[[Category: Piscotta, F J]]
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[[Category: Piscotta FJ]]
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[[Category: Adp-ribosyltransferase]]
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[[Category: Parst]]
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[[Category: Toxin]]
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[[Category: Toxin-antitoxin complex]]
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Current revision

ParT: Prs ADP-ribosylating toxin bound to cognate antitoxin ParS

PDB ID 6d0h

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