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1zci

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(New page: 200px<br /> <applet load="1zci" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zci, resolution 1.65&Aring;" /> '''HIV-1 DIS RNA subty...)
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[[Image:1zci.gif|left|200px]]<br />
 
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<applet load="1zci" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1zci, resolution 1.65&Aring;" />
 
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'''HIV-1 DIS RNA subtype F- monoclinic form'''<br />
 
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==Overview==
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==HIV-1 DIS RNA subtype F- monoclinic form==
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All retroviruses encapsidate their genome as a dimer of homologous, single-stranded RNAs. The dimerization initiation site (DIS) of human, immunodeficiency virus type 1 (HIV-1) is located in the 5'-untranslated, region of the viral genome and consists of a hairpin with a 6 nt, self-complementary loop sequence. Genomic RNA dimerization, a crucial step, for virion infectivity, is promoted by the formation of a loop-loop, complex (or kissing complex) between two DIS hairpins. Crystal structures, for the subtypes A, B and F of the HIV-1 DIS kissing complex have now been, solved at 2.3 A, 1.9 A and 1.6 A, respectively. They revealed a, polymorphism of bulged-out residues showing clearly that their, conformation is not a mere consequence of crystal packing. They also, provide more insights into ion binding, hydration, and RNA conformation, and flexibility. In particular, we observed the binding of spermine to the, loop-loop helix, which displaced a magnesium cation important for subtype, A DIS dimerization. The excellent agreement between X-ray structures and, the results of chemical probing and interference data on larger viral RNA, fragments shows that the crystal structures are relevant for the DIS, kissing complex present in solution and in viral particles. Accordingly, these structures will be helpful for designing new drugs derived from, aminoglycoside antibiotics and targeted against the RNA dimerization step, of the viral life-cycle.
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<StructureSection load='1zci' size='340' side='right'caption='[[1zci]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1zci]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZCI FirstGlance]. <br>
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1ZCI is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with K as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1ZCI OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5BU:5-BROMO-URIDINE-5-MONOPHOSPHATE'>5BU</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zci OCA], [https://pdbe.org/1zci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zci RCSB], [https://www.ebi.ac.uk/pdbsum/1zci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zci ProSAT]</span></td></tr>
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Polymorphism of bulged-out residues in HIV-1 RNA DIS kissing complex and structure comparison with solution studies., Ennifar E, Dumas P, J Mol Biol. 2006 Feb 24;356(3):771-82. Epub 2005 Dec 21. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=16403527 16403527]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: Dumas, P.]]
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</StructureSection>
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[[Category: Ennifar, E.]]
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[[Category: Large Structures]]
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[[Category: K]]
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[[Category: Dumas P]]
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[[Category: hiv-1]]
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[[Category: Ennifar E]]
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[[Category: loop-loop complex]]
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[[Category: metal ions]]
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[[Category: rna]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 14:38:29 2007''
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Current revision

HIV-1 DIS RNA subtype F- monoclinic form

PDB ID 1zci

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