Terminase
From Proteopedia
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<StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'> | <StructureSection load='4ife' size='350' side='right' caption='Terminase large subunit complex with ATP (PDB code [[4ife]])' scene='77/775254/Cv/1'> | ||
| - | + | __TOC__ | |
== Function == | == Function == | ||
| - | '''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits (TRM1, TRM2 and TRM3), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>. | + | '''Terminase''' (Ter) is a key component of the DNA packaging machine found in bacteriophages and herpesviruses. The Ter complex is comprised of a '''small Ter subunit''' which is a recognition subunit and a '''large Ter subunit''' which is an endonuclease/translocase subunit <ref>PMID:22'''297528</ref>. The nuclease activity of the large Ter subunit is stimulated by ATP. The '''tripartite terminase''' complex of herpesvirus which contains 3 subunits ('''TRM1, TRM2 and TRM3'''), is found in the cytoplasm of infected cells and uses the cell's import machinery to enter the nucleus<ref>PMID:27033706</ref>. '''TRM3''' has RNase H-like activity that plays an important role for the cleavage of concatemeric viral DNA into unit length genome<ref>PMID:24155374</ref>. |
== Structural highlights == | == Structural highlights == | ||
The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>. | The large subunit of Ter is composed of an <scene name='77/775254/Cv/5'>N-terminal ATPase domain, a linker region and a C-terminal nuclease domain</scene>. ATP binds to the protein in a <scene name='77/775254/Cv/6'>groove between the ATPase domain and the linker region</scene><ref>PMID:23630261</ref>. | ||
| + | == 3D Structures of terminase == | ||
| + | [[Terminase 3D Structures ]] | ||
</StructureSection> | </StructureSection> | ||
| - | == 3D Structures of terminase == | ||
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| - | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
| - | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
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| - | *Terminase small subunit | ||
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| - | **[[3n4p]], [[3n4q]] – HvTer nuclease domain – Herpesvirus<br /> | ||
| - | **[[6ey7]] – HvTer nuclease domain + inhibitor<br /> | ||
| - | **[[3hef]] – Ter – Bacteriophage T7 <br /> | ||
| - | **[[4zc3]], [[2cmp]] – Sf6Ter nuclease domain – Bacteriophage Sf6 <br /> | ||
| - | **[[4dyc]], [[4dzj]], [[4dzp]] – Sf6Ter nuclease domain (mutant) <br /> | ||
| - | **[[3zqp]], [[3zqm]], [[3zqn]], [[3zqo]] – Sf6Ter oligomerization domain <br /> | ||
| - | **[[3zqq]], [[4dyq]], [[4dyr]] – Sf6Ter <br /> | ||
| - | **[[1j9i]] – Ter nuclease domain – Bacteriophage λ - NMR <br /> | ||
| - | **[[4xvn]] – G20cTer nuclease domain – Bacteriophage G20c <br /> | ||
| - | **[[3p9a]] – Ter – Bacteriophage P22 <br /> | ||
| - | **[[6v1i]] – Ter – Thermus virus – Cryo EM<br /> | ||
| - | **[[6ejq]] – Ter – Thermus phage<br /> | ||
| - | **[[6hn7]] – Ter + RPPC – ''E. coli'' virus λ <br /> | ||
| - | **[[7joq]] – Ter – Pseudomonas phage NV1<br /> | ||
| - | **[[6w7t]] – Ter – Pseudomonas phage PaP3<br /> | ||
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| - | *Terminase large subunit | ||
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| - | **[[4dkw]] – Ter nuclease domain – Bacteriophage P22<br /> | ||
| - | **[[4bij]], [[4bil]] – T7Ter – Bacteriophage T7 – Cryo EM<br /> | ||
| - | **[[4idh]] – T7Ter <br /> | ||
| - | **[[2wbn]], [[2wc9]] – Ter nuclease domain – Bacteriophage Spp1 <br /> | ||
| - | **[[3c6a]], [[3c6h]] – Ter nuclease domain – Bacteriophage Rb49 <br /> | ||
| - | **[[5c10]], [[5c12]] – Sf6Ter nuclease domain <br /> | ||
| - | **[[5c2d]], [[5c15]] – Sf6Ter nuclease domain (mutant)<br /> | ||
| - | **[[5m1f]], [[5m1k]], [[5m1n]], [[5m1o]], [[5m1p]], [[5m1q]] – G20cTer <br /> | ||
| - | **[[5oe8]], [[5oee]], [[5oe9]] – D6eTer – Bacteriophage D6e <br /> | ||
| - | **[[5tge]] – P74Ter nuclease domain – Thermus phage P74 <br /> | ||
| - | **[[4zni]], [[4znk]] – P74Ter ATPase domain <br /> | ||
| - | **[[4znj]] – P74Ter ATPase domain (mutant)<br /> | ||
| - | **[[4znl]] – P74Ter ATPase domain + ADP <br /> | ||
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| - | *Terminase large subunit complex | ||
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| - | **[[4iee]], [[4ife]] – Sf6Ter + ATP<br /> | ||
| - | **[[4iei]] – Sf6Ter + ADP<br /> | ||
| - | **[[5c2f]] – Sf6Ter nuclease domain (mutant) + antifungal drug<br /> | ||
| - | **[[5oea]] – D6eTer + ATP <br /> | ||
| - | **[[5oeb]] – D6eTer + ADP <br /> | ||
| - | **[[6vi1]], [[6xmi]] – Ter + antibody – Salmonella phage P22<br /> | ||
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| - | *Tripartite terminase | ||
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| - | **[[6m5s]], [[6m5r]] – HvTer TRM1+TRM2+TRM3 – Cryo EM<br /> | ||
| - | **[[6m5u]], [[6m5v]] – HvTer TRM1+TRM2+TRM3 + ADP – Cryo EM<br /> | ||
| - | **[[6m5t]] – HvTer TRM3 – Cryo EM<br /> | ||
| - | }} | ||
== References == | == References == | ||
<references/> | <references/> | ||
[[Category:Topic Page]] | [[Category:Topic Page]] | ||
Current revision
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References
- ↑ PMID:22297528
- ↑ Sankhala RS, Lokareddy RK, Cingolani G. Divergent Evolution of Nuclear Localization Signal Sequences in Herpesvirus Terminase Subunits. J Biol Chem. 2016 Mar 31. pii: jbc.M116.724393. PMID:27033706 doi:http://dx.doi.org/10.1074/jbc.M116.724393
- ↑ Heming JD, Huffman JB, Jones LM, Homa FL. Isolation and characterization of the herpes simplex virus 1 terminase complex. J Virol. 2014 Jan;88(1):225-36. doi: 10.1128/JVI.02632-13. Epub 2013 Oct 23. PMID:24155374 doi:http://dx.doi.org/10.1128/JVI.02632-13
- ↑ Zhao H, Christensen TE, Kamau YN, Tang L. Structures of the phage Sf6 large terminase provide new insights into DNA translocation and cleavage. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8075-8080. Epub 2013 Apr 29. PMID:23630261 doi:10.1073/pnas.1301133110
