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| | <StructureSection load='6vci' size='340' side='right'caption='[[6vci]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='6vci' size='340' side='right'caption='[[6vci]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6vci]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VCI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VCI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6vci]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VCI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VCI FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">yebT, b1834, JW1823 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vci OCA], [http://pdbe.org/6vci PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vci RCSB], [http://www.ebi.ac.uk/pdbsum/6vci PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vci ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vci FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vci OCA], [https://pdbe.org/6vci PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vci RCSB], [https://www.ebi.ac.uk/pdbsum/6vci PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vci ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/YEBT_ECOLI YEBT_ECOLI]] Component of a transport pathway that contributes to membrane integrity (PubMed:27795327). May directly span the intermembrane space, facilitating the transport of substrates across the periplasm (Probable).<ref>PMID:27795327</ref> <ref>PMID:28388411</ref> | + | [https://www.uniprot.org/uniprot/YEBT_ECOLI YEBT_ECOLI] Component of a transport pathway that contributes to membrane integrity (PubMed:27795327). May directly span the intermembrane space, facilitating the transport of substrates across the periplasm (Probable).<ref>PMID:27795327</ref> <ref>PMID:28388411</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Ecoli]] | + | [[Category: Escherichia coli K-12]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bhabha, G]] | + | [[Category: Bhabha G]] |
| - | [[Category: Coudray, N]] | + | [[Category: Coudray N]] |
| - | [[Category: Ekiert, D C]] | + | [[Category: Ekiert DC]] |
| - | [[Category: Isom, G L]] | + | [[Category: Isom GL]] |
| - | [[Category: MacRae, M R]] | + | [[Category: MacRae MR]] |
| - | [[Category: McManus, C T]] | + | [[Category: McManus CT]] |
| - | [[Category: Bacterial cell envelope]]
| + | |
| - | [[Category: Letb]]
| + | |
| - | [[Category: Lipid transport]]
| + | |
| - | [[Category: Mce]]
| + | |
| - | [[Category: Yebt]]
| + | |
| Structural highlights
Function
YEBT_ECOLI Component of a transport pathway that contributes to membrane integrity (PubMed:27795327). May directly span the intermembrane space, facilitating the transport of substrates across the periplasm (Probable).[1] [2]
Publication Abstract from PubMed
Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 A cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel sufficiently long to span the periplasm. Lipids bind inside the tunnel, suggesting that it functions as a pathway for lipid transport. Cryo-EM structures in the open and closed states reveal a dynamic tunnel lining, with implications for gating or substrate translocation. Our results support a model in which LetB establishes a physical link between the two membranes and creates a hydrophobic pathway for the translocation of lipids across the periplasm.
LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.,Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G Cell. 2020 Apr 30;181(3):653-664.e19. doi: 10.1016/j.cell.2020.03.030. PMID:32359438[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nakayama T, Zhang-Akiyama QM. pqiABC and yebST, Putative mce Operons of Escherichia coli, Encode Transport Pathways and Contribute to Membrane Integrity. J Bacteriol. 2016 Dec 13;199(1). pii: JB.00606-16. doi: 10.1128/JB.00606-16., Print 2017 Jan 1. PMID:27795327 doi:http://dx.doi.org/10.1128/JB.00606-16
- ↑ Ekiert DC, Bhabha G, Isom GL, Greenan G, Ovchinnikov S, Henderson IR, Cox JS, Vale RD. Architectures of Lipid Transport Systems for the Bacterial Outer Membrane. Cell. 2017 Apr 6;169(2):273-285.e17. doi: 10.1016/j.cell.2017.03.019. PMID:28388411 doi:http://dx.doi.org/10.1016/j.cell.2017.03.019
- ↑ Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G. LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope. Cell. 2020 Apr 30;181(3):653-664.e19. doi: 10.1016/j.cell.2020.03.030. PMID:32359438 doi:http://dx.doi.org/10.1016/j.cell.2020.03.030
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