|
|
| Line 3: |
Line 3: |
| | <StructureSection load='6yir' size='340' side='right'caption='[[6yir]], [[Resolution|resolution]] 1.68Å' scene=''> | | <StructureSection load='6yir' size='340' side='right'caption='[[6yir]], [[Resolution|resolution]] 1.68Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6yir]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YIR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YIR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yir]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YIR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YIR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.68Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">msmX, yxkG, BSU38810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yir OCA], [http://pdbe.org/6yir PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yir RCSB], [http://www.ebi.ac.uk/pdbsum/6yir PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yir ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yir FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yir OCA], [https://pdbe.org/6yir PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yir RCSB], [https://www.ebi.ac.uk/pdbsum/6yir PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yir ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MSMX_BACSU MSMX_BACSU]] Part of the ABC transporter complex involved in maltodextrin import (Probable). Is also part of the ABC transporter complex MelEDC-MsmX involved in melibiose, raffinose and stachyose import (PubMed:31138628). Probably responsible for energy coupling to the transport system (Probable).<ref>PMID:31138628</ref> <ref>PMID:16707683</ref> | + | [https://www.uniprot.org/uniprot/MSMX_BACSU MSMX_BACSU] Part of the ABC transporter complex involved in maltodextrin import (Probable). Is also part of the ABC transporter complex MelEDC-MsmX involved in melibiose, raffinose and stachyose import (PubMed:31138628). Probably responsible for energy coupling to the transport system (Probable).<ref>PMID:31138628</ref> <ref>PMID:16707683</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 23: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacsu]] | + | [[Category: Bacillus subtilis subsp. subtilis str. 168]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leisico, F]] | + | [[Category: Leisico F]] |
| - | [[Category: Romao, M J]] | + | [[Category: Romao MJ]] |
| - | [[Category: Santos-Silva, T]] | + | [[Category: Santos-Silva T]] |
| - | [[Category: Atpase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Multitask]]
| + | |
| - | [[Category: Polysaccharide import]]
| + | |
| Structural highlights
Function
MSMX_BACSU Part of the ABC transporter complex involved in maltodextrin import (Probable). Is also part of the ABC transporter complex MelEDC-MsmX involved in melibiose, raffinose and stachyose import (PubMed:31138628). Probably responsible for energy coupling to the transport system (Probable).[1] [2]
Publication Abstract from PubMed
ATP-binding cassette (ABC) type I importers are widespread in bacteria and play a crucial role in its survival and pathogenesis. They share the same modular architecture comprising two intracellular nucleotide-binding domains (NBDs), two transmembrane domains (TMDs) and a substrate-binding protein. The NBDs bind and hydrolyze ATP, thereby generating conformational changes that are coupled to the TMDs and lead to substrate translocation. A group of multitask NBDs that are able to serve as the cellular motor for multiple sugar importers was recently discovered. To understand why some ABC importers share energy-coupling components, we used the MsmX ATPase from Bacillus subtilis as a model for biological and structural studies. Here we report the first examples of functional hybrid interspecies ABC type I importers in which the NBDs could be exchanged. Furthermore, the first crystal structure of an assigned multitask NBD provides a framework to understand the molecular basis of the broader specificity of interaction with the TMDs.
Multitask ATPases (NBDs) of bacterial ABC importers type I and their interspecies exchangeability.,Leisico F, Godinho LM, Goncalves IC, Silva SP, Carneiro B, Romao MJ, Santos-Silva T, de Sa-Nogueira I Sci Rep. 2020 Nov 11;10(1):19564. doi: 10.1038/s41598-020-76444-0. PMID:33177617[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Morabbi Heravi K, Watzlawick H, Altenbuchner J. The melREDCA Operon Encodes a Utilization System for the Raffinose Family of Oligosaccharides in Bacillus subtilis. J Bacteriol. 2019 Jul 10;201(15). pii: JB.00109-19. doi: 10.1128/JB.00109-19., Print 2019 Aug 1. PMID:31138628 doi:http://dx.doi.org/10.1128/JB.00109-19
- ↑ Schonert S, Seitz S, Krafft H, Feuerbaum EA, Andernach I, Witz G, Dahl MK. Maltose and maltodextrin utilization by Bacillus subtilis. J Bacteriol. 2006 Jun;188(11):3911-22. PMID:16707683 doi:http://dx.doi.org/188/11/3911
- ↑ Leisico F, Godinho LM, Goncalves IC, Silva SP, Carneiro B, Romao MJ, Santos-Silva T, de Sa-Nogueira I. Multitask ATPases (NBDs) of bacterial ABC importers type I and their interspecies exchangeability. Sci Rep. 2020 Nov 11;10(1):19564. doi: 10.1038/s41598-020-76444-0. PMID:33177617 doi:http://dx.doi.org/10.1038/s41598-020-76444-0
|
