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| | <StructureSection load='7a46' size='340' side='right'caption='[[7a46]], [[Resolution|resolution]] 3.00Å' scene=''> | | <StructureSection load='7a46' size='340' side='right'caption='[[7a46]], [[Resolution|resolution]] 3.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7a46]] is a 7 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A46 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7A46 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7a46]] is a 7 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7A46 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7A46 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ybiO, BN17_06071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7a46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a46 OCA], [https://pdbe.org/7a46 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7a46 RCSB], [https://www.ebi.ac.uk/pdbsum/7a46 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7a46 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7a46 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7a46 OCA], [http://pdbe.org/7a46 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7a46 RCSB], [http://www.ebi.ac.uk/pdbsum/7a46 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7a46 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/YBIO_ECOLI YBIO_ECOLI] Mechanosensitive channel that protects cells against hypoosmotic stress when highly overexpressed.<ref>PMID:22874652</ref> |
| - | The mechanosensitive channel of small conductance (MscS) is the prototype of an evolutionarily diversified large family that fine-tunes osmoregulation but is likely to fulfill additional functions. Escherichia coli has six osmoprotective paralogs with different numbers of transmembrane helices. These helices are important for gating and sensing in MscS but the role of the additional helices in the paralogs is not understood. The medium-sized channel YnaI was extracted and delivered in native nanodiscs in closed-like and open-like conformations using the copolymer diisobutylene/maleic acid (DIBMA) for structural studies. Here we show by electron cryomicroscopy that YnaI has an extended sensor paddle that during gating relocates relative to the pore concomitant with bending of a GGxGG motif in the pore helices. YnaI is the only one of the six paralogs that has this GGxGG motif allowing the sensor paddle to move outward. Access to the pore is through a vestibule on the cytosolic side that is fenestrated by side portals. In YnaI, these portals are obstructed by aromatic side chains but are still fully hydrated and thus support conductance. For comparison with large-sized channels, we determined the structure of YbiO, which showed larger portals and a wider pore with no GGxGG motif. Further in silico comparison of MscS, YnaI, and YbiO highlighted differences in the hydrophobicity and wettability of their pores and vestibule interiors. Thus, MscS-like channels of different sizes have a common core architecture but show different gating mechanisms and fine-tuned conductive properties.
| + | |
| - | | + | |
| - | The MscS-like channel YnaI has a gating mechanism based on flexible pore helices.,Flegler VJ, Rasmussen A, Rao S, Wu N, Zenobi R, Sansom MSP, Hedrich R, Rasmussen T, Bottcher B Proc Natl Acad Sci U S A. 2020 Nov 4. pii: 2005641117. doi:, 10.1073/pnas.2005641117. PMID:33148804<ref>PMID:33148804</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 7a46" style="background-color:#fffaf0;"></div>
| + | |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boettcher, B]] | + | [[Category: Boettcher B]] |
| - | [[Category: Flegler, V J]] | + | [[Category: Flegler VJ]] |
| - | [[Category: Hedrich, R]] | + | [[Category: Hedrich R]] |
| - | [[Category: Rao, S]] | + | [[Category: Rao S]] |
| - | [[Category: Rasmussen, A]] | + | [[Category: Rasmussen A]] |
| - | [[Category: Rasmussen, T]] | + | [[Category: Rasmussen T]] |
| - | [[Category: Sansom, M S.P]] | + | [[Category: Sansom MSP]] |
| - | [[Category: Wu, N]] | + | [[Category: Wu N]] |
| - | [[Category: Zenobi, R]] | + | [[Category: Zenobi R]] |
| - | [[Category: E. coli]]
| + | |
| - | [[Category: Mechanosensitive channel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Mscs-like channel]]
| + | |
| - | [[Category: Ybio]]
| + | |
