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| | <StructureSection load='1jv7' size='340' side='right'caption='[[1jv7]], [[Resolution|resolution]] 2.25Å' scene=''> | | <StructureSection load='1jv7' size='340' side='right'caption='[[1jv7]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jv7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_halobius_ruber"_klebahn_1919 "bacillus halobius ruber" klebahn 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1JV7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jv7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JV7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JV7 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1jv6|1jv6]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LI1:1-[2,6,10.14-TETRAMETHYL-HEXADECAN-16-YL]-2-[2,10,14-TRIMETHYLHEXADECAN-16-YL]GLYCEROL'>LI1</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Bop ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2242 "Bacillus halobius ruber" Klebahn 1919])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jv7 OCA], [https://pdbe.org/1jv7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jv7 RCSB], [https://www.ebi.ac.uk/pdbsum/1jv7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jv7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1jv7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jv7 OCA], [http://pdbe.org/1jv7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jv7 RCSB], [http://www.ebi.ac.uk/pdbsum/1jv7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jv7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA]] Light-driven proton pump. | + | [https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus halobius ruber klebahn 1919]] | + | [[Category: Halobacterium salinarum]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cartailler, J P]] | + | [[Category: Cartailler J-P]] |
| - | [[Category: Facciotti, M T]] | + | [[Category: Facciotti MT]] |
| - | [[Category: Glaeser, R M]] | + | [[Category: Glaeser RM]] |
| - | [[Category: Lanyi, J K]] | + | [[Category: Lanyi JK]] |
| - | [[Category: Luecke, H]] | + | [[Category: Luecke H]] |
| - | [[Category: Needleman, R]] | + | [[Category: Needleman R]] |
| - | [[Category: Rouhani, S]] | + | [[Category: Rouhani S]] |
| - | [[Category: Walian, P]] | + | [[Category: Walian P]] |
| - | [[Category: 7-transmembrane]]
| + | |
| - | [[Category: Cubic lipid phase]]
| + | |
| - | [[Category: D85s mutant]]
| + | |
| - | [[Category: Haloarchaea]]
| + | |
| - | [[Category: Ion transport]]
| + | |
| - | [[Category: O-like state]]
| + | |
| - | [[Category: Photocycle intermediate]]
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| - | [[Category: Photoreceptor]]
| + | |
| Structural highlights
Function
BACR_HALSA Light-driven proton pump.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Crystal structures are reported for the D85S and D85S/F219L mutants of the light-driven proton/hydroxyl-pump bacteriorhodopsin. These mutants crystallize in the orthorhombic C222(1) spacegroup, and provide the first demonstration that monoolein-based cubic lipid phase crystallization can support the growth of well-diffracting crystals in non-hexagonal spacegroups. Both structures exhibit similar and substantial differences relative to wild-type bacteriorhodopsin, suggesting that they represent inherent features resulting from neutralization of the Schiff base counterion Asp85. We argue that these structures provide a model for the last photocycle intermediate (O) of bacteriorhodopsin, in which Asp85 is protonated, the proton release group is deprotonated, and the retinal has reisomerized to all-trans. Unlike for the M and N photointermediates, where structural changes occur mainly on the cytoplasmic side, here the large-scale changes are confined to the extracellular side. As in the M intermediate, the side-chain of Arg82 is in a downward configuration, and in addition, a pi-cloud hydrogen bond forms between Trp189 NE1 and Trp138. On the cytoplasmic side, there is increased hydration near the surface, suggesting how Asp96 might communicate with the bulk during the rise of the O intermediate.
Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate.,Rouhani S, Cartailler JP, Facciotti MT, Walian P, Needleman R, Lanyi JK, Glaeser RM, Luecke H J Mol Biol. 2001 Oct 26;313(3):615-28. PMID:11676543[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rouhani S, Cartailler JP, Facciotti MT, Walian P, Needleman R, Lanyi JK, Glaeser RM, Luecke H. Crystal structure of the D85S mutant of bacteriorhodopsin: model of an O-like photocycle intermediate. J Mol Biol. 2001 Oct 26;313(3):615-28. PMID:11676543 doi:10.1006/jmbi.2001.5066
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