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| | ==Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor== | | ==Solution Structure of the DNA-Binding Domain of Interleukin Enhancer Binding Factor== |
| - | <StructureSection load='1jxs' size='340' side='right'caption='[[1jxs]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1jxs' size='340' side='right'caption='[[1jxs]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1jxs]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1JXS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1jxs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1JXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1JXS FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ILF-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jxs OCA], [http://pdbe.org/1jxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1jxs RCSB], [http://www.ebi.ac.uk/pdbsum/1jxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1jxs ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1jxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1jxs OCA], [https://pdbe.org/1jxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1jxs RCSB], [https://www.ebi.ac.uk/pdbsum/1jxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1jxs ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FOXK2_HUMAN FOXK2_HUMAN]] Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.<ref>PMID:1909027</ref> <ref>PMID:1339390</ref> | + | [https://www.uniprot.org/uniprot/FOXK2_HUMAN FOXK2_HUMAN] Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.<ref>PMID:1909027</ref> <ref>PMID:1339390</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chen, S H]] | + | [[Category: Chen SH]] |
| - | [[Category: Chen, S W]] | + | [[Category: Chen SW]] |
| - | [[Category: Chuang, W J]] | + | [[Category: Chuang WJ]] |
| - | [[Category: Hsieh, Y H]] | + | [[Category: Hsieh YH]] |
| - | [[Category: Jeng, W Y]] | + | [[Category: Jeng WY]] |
| - | [[Category: Li, C]] | + | [[Category: Li C]] |
| - | [[Category: Liu, P P]] | + | [[Category: Liu PP]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Dna-binding domain]]
| + | |
| - | [[Category: Winged helix]]
| + | |
| Structural highlights
Function
FOXK2_HUMAN Recognizes the core sequence 5'-TAAACA-3'. Binds to NFAT-like motifs (purine-rich) in the IL2 promoter. Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Interleukin enhancer binding factor (ILF) binds to the interleukin-2 (IL-2) promoter and regulates IL-2 gene expression. In this study, the 3D structure of the DNA-binding domain of ILF was determined by multidimensional NMR spectroscopy. NMR structure analysis revealed that the DNA-binding domain of ILF is a new member of the winged helix/forkhead family, and that its wing 2 contains an extra alpha-helix. This is the first study to report the presence of a C-terminal alpha-helix in place of a typical wing 2 in a member of this family. This structural difference may be responsible for the different DNA-binding specificity of ILF compared to other winged helix/forkhead proteins. Our deletion studies of the fragments of ILF also suggest that the C-terminal region plays a regulatory role in DNA binding.
Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a).,Liu PP, Chen YC, Li C, Hsieh YH, Chen SW, Chen SH, Jeng WY, Chuang WJ Proteins. 2002 Dec 1;49(4):543-53. PMID:12402362[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li C, Lai CF, Sigman DS, Gaynor RB. Cloning of a cellular factor, interleukin binding factor, that binds to NFAT-like motifs in the human immunodeficiency virus long terminal repeat. Proc Natl Acad Sci U S A. 1991 Sep 1;88(17):7739-43. PMID:1909027
- ↑ Li C, Lusis AJ, Sparkes R, Nirula A, Gaynor R. Characterization and chromosomal mapping of the gene encoding the cellular DNA binding protein ILF. Genomics. 1992 Jul;13(3):665-71. PMID:1339390
- ↑ Liu PP, Chen YC, Li C, Hsieh YH, Chen SW, Chen SH, Jeng WY, Chuang WJ. Solution structure of the DNA-binding domain of interleukin enhancer binding factor 1 (FOXK1a). Proteins. 2002 Dec 1;49(4):543-53. PMID:12402362 doi:10.1002/prot.10227
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