6yyh
From Proteopedia
(Difference between revisions)
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
==Crystal structure of beta-D-xylosidase from Dictyoglomus thermophilum in ligand-free form== | ==Crystal structure of beta-D-xylosidase from Dictyoglomus thermophilum in ligand-free form== | ||
| - | <StructureSection load='6yyh' size='340' side='right'caption='[[6yyh]]' scene=''> | + | <StructureSection load='6yyh' size='340' side='right'caption='[[6yyh]], [[Resolution|resolution]] 2.72Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YYH OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6yyh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyoglomus_thermophilum_H-6-12 Dictyoglomus thermophilum H-6-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YYH FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.72Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yyh OCA], [https://pdbe.org/6yyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yyh RCSB], [https://www.ebi.ac.uk/pdbsum/6yyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yyh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/B5YB78_DICT6 B5YB78_DICT6] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Dictyoglomus thermophilum beta-d-xylosidase DtXyl is attractive as a potential thermostable biocatalyst able to produce biologically active ginsenosides intermediates from beta-(1,2)-D-xylosylated compounds, including Notoginsenoside-R1. DtXyl was expressed as an active N-terminal His-tagged protein, and its crystal structure was solved in presence or absence of d-xylose product. Modelling of notoginsenoside R1 in DtXyl active site led to the identification of several hydrophobic residues interacting in close contact to the substrate hydrophobic core. Unlike other residues involved in substrate binding, these residues are not conserved among GH39 xylosidase family, and their physico-chemical properties can be correlated to the efficient binding and subsequent hydrolysis of Notoginsenoside R1. | ||
| + | |||
| + | Crystal structure of Dictyoglomus thermophilum beta-d-xylosidase DtXyl unravels the structural determinants for efficient notoginsenoside R1 hydrolysis.,Bretagne D, Paris A, de Vaumas R, Lafite P, Daniellou R Biochimie. 2020 Nov 23;181:34-41. doi: 10.1016/j.biochi.2020.11.017. PMID:33242495<ref>PMID:33242495</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6yyh" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Xylosidase 3D structures|Xylosidase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Dictyoglomus thermophilum H-6-12]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Bretagne D]] | [[Category: Bretagne D]] | ||
[[Category: Daniellou R]] | [[Category: Daniellou R]] | ||
[[Category: Lafite P]] | [[Category: Lafite P]] | ||
Current revision
Crystal structure of beta-D-xylosidase from Dictyoglomus thermophilum in ligand-free form
| |||||||||||
