1mbm
From Proteopedia
(Difference between revisions)
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<StructureSection load='1mbm' size='340' side='right'caption='[[1mbm]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='1mbm' size='340' side='right'caption='[[1mbm]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[1mbm]] is a 4 chain structure with sequence from [ | + | <table><tr><td colspan='2'>[[1mbm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Equine_arteritis_virus Equine arteritis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MBM FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mbm OCA], [https://pdbe.org/1mbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mbm RCSB], [https://www.ebi.ac.uk/pdbsum/1mbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mbm ProSAT]</span></td></tr> | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/RPOA_EAVBU RPOA_EAVBU] The replicase polyprotein 1ab is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products.<ref>PMID:18078692</ref> Nsp1 is essential for viral subgenomic mRNA synthesis.<ref>PMID:18078692</ref> Nsp2 cysteine proteinase which cleaves the nsp2/nsp3 site in the polyprotein. Also displays deubiquitinating and deISGylase activities. The deubiquitinating activity cleaves both ubiquitinated and ISGylated products and may therefore regulate ubiquitin and ISG15 dependent host innate immunity.<ref>PMID:18078692</ref> The 3C-like serine proteinase chain is responsible for the majority of cleavages as it cleaves the C-terminus of the polyprotein.<ref>PMID:18078692</ref> The helicase chain, which contains a zinc finger structure, displays RNA and DNA duplex-unwinding activities with 5' to 3' polarity.<ref>PMID:18078692</ref> |
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mbm ConSurf]. | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mbm ConSurf]. | ||
<div style="clear:both"></div> | <div style="clear:both"></div> | ||
| - | <div style="background-color:#fffaf0;"> | ||
| - | == Publication Abstract from PubMed == | ||
| - | Arteriviruses are enveloped, positive-stranded RNA viruses and include pathogens of major economic concern to the swine- and horse-breeding industries. The arterivirus replicase gene encodes two large precursor polyproteins that are processed by the viral main proteinase nonstructural protein 4 (nsp4). The three-dimensional structure of the 21-kDa nsp4 from the arterivirus prototype equine arteritis virus has been determined to 2.0 A resolution. Nsp4 adopts the smallest known chymotrypsin-like fold with a canonical catalytic triad of Ser-120, His-39, and Asp-65, as well as a novel alpha/beta C-terminal extension domain that may play a role in mediating protein-protein interactions. In different copies of nsp4 in the asymmetric unit, the oxyanion hole adopts either a collapsed inactive conformation or the standard active conformation, which may be a novel way of regulating proteolytic activity. | ||
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| - | Structure of arterivirus nsp4. The smallest chymotrypsin-like proteinase with an alpha/beta C-terminal extension and alternate conformations of the oxyanion hole.,Barrette-Ng IH, Ng KK, Mark BL, Van Aken D, Cherney MM, Garen C, Kolodenko Y, Gorbalenya AE, Snijder EJ, James MN J Biol Chem. 2002 Oct 18;277(42):39960-6. Epub 2002 Aug 5. PMID:12163505<ref>PMID:12163505</ref> | ||
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| - | </div> | ||
| - | <div class="pdbe-citations 1mbm" style="background-color:#fffaf0;"></div> | ||
==See Also== | ==See Also== | ||
| - | *[[Proteinase|Proteinase]] | ||
*[[Proteinase 3D structures|Proteinase 3D structures]] | *[[Proteinase 3D structures|Proteinase 3D structures]] | ||
== References == | == References == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| - | [[Category: | + | [[Category: Equine arteritis virus]] |
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | + | [[Category: Barrette-Ng IH]] | |
| - | [[Category: Barrette-Ng | + | [[Category: Cherney MM]] |
| - | [[Category: Cherney | + | [[Category: Garen C]] |
| - | [[Category: Garen | + | [[Category: Gorbalenya AE]] |
| - | [[Category: Gorbalenya | + | [[Category: James MNG]] |
| - | [[Category: James | + | [[Category: Kolodenko Y]] |
| - | [[Category: Kolodenko | + | [[Category: Mark BL]] |
| - | [[Category: Mark | + | [[Category: Ng KK-S]] |
| - | [[Category: Ng | + | [[Category: Snijder EJ]] |
| - | [[Category: Snijder | + | [[Category: Van Aken D]] |
| - | [[Category: | + | |
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Current revision
NSP4 proteinase from Equine Arteritis Virus
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