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| | <StructureSection load='1mij' size='340' side='right'caption='[[1mij]], [[Resolution|resolution]] 2.05Å' scene=''> | | <StructureSection load='1mij' size='340' side='right'caption='[[1mij]], [[Resolution|resolution]] 2.05Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1mij]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1MIJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1mij]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MIJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MIJ FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.05Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PROS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1mij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mij OCA], [http://pdbe.org/1mij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1mij RCSB], [http://www.ebi.ac.uk/pdbsum/1mij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1mij ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mij OCA], [https://pdbe.org/1mij PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mij RCSB], [https://www.ebi.ac.uk/pdbsum/1mij PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mij ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PROS_DROME PROS_DROME]] Required for proper neuronal differentiation of most or all neurons and their precursors in central and peripheral nervous systems, axonal outgrowth and pathfinding. Not required for the specification of neuronal identity. May regulate transcription by binding to DNA.<ref>PMID:1720353</ref> <ref>PMID:1842358</ref> <ref>PMID:1540176</ref> <ref>PMID:11051550</ref> | + | [https://www.uniprot.org/uniprot/PROS_DROME PROS_DROME] Required for proper neuronal differentiation of most or all neurons and their precursors in central and peripheral nervous systems, axonal outgrowth and pathfinding. Not required for the specification of neuronal identity. May regulate transcription by binding to DNA.<ref>PMID:1720353</ref> <ref>PMID:1842358</ref> <ref>PMID:1540176</ref> <ref>PMID:11051550</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mij_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mi/1mij_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Doe, C Q]] | + | [[Category: Doe CQ]] |
| - | [[Category: Matthews, B W]] | + | [[Category: Matthews BW]] |
| - | [[Category: Ryter, J M]] | + | [[Category: Ryter JM]] |
| - | [[Category: 4-helix bundle]]
| + | |
| - | [[Category: Dna-binding domain]]
| + | |
| - | [[Category: Homeodomain]]
| + | |
| - | [[Category: Prospero domain]]
| + | |
| - | [[Category: Transcription]]
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| Structural highlights
Function
PROS_DROME Required for proper neuronal differentiation of most or all neurons and their precursors in central and peripheral nervous systems, axonal outgrowth and pathfinding. Not required for the specification of neuronal identity. May regulate transcription by binding to DNA.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Prospero transcription factor promotes neural differentiation in Drosophila, and its activity is tightly regulated by modulating its subcellular localization. Prospero is exported from the nucleus of neural precursors but imported into the nucleus of daughter cells, which is necessary for their proper differentiation. Prospero has a highly divergent putative homeodomain adjacent to a conserved Prospero domain; both are required for sequence-specific DNA binding. Here we show that the structure of these two regions consists of a single structural unit (a homeo-prospero domain), in which the Prospero domain region is in position to contribute to DNA binding and also to mask a defined nuclear export signal that is within the putative homeodomain region. We propose that the homeo-prospero domain coordinately regulates Prospero nuclear localization and DNA binding specificity.
Structure of the DNA binding region of prospero reveals a novel homeo-prospero domain.,Ryter JM, Doe CQ, Matthews BW Structure. 2002 Nov;10(11):1541-9. PMID:12429095[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vaessin H, Grell E, Wolff E, Bier E, Jan LY, Jan YN. prospero is expressed in neuronal precursors and encodes a nuclear protein that is involved in the control of axonal outgrowth in Drosophila. Cell. 1991 Nov 29;67(5):941-53. PMID:1720353
- ↑ Chu-Lagraff Q, Wright DM, McNeil LK, Doe CQ. The prospero gene encodes a divergent homeodomain protein that controls neuronal identity in Drosophila. Development. 1991;Suppl 2:79-85. PMID:1842358
- ↑ Matsuzaki F, Koizumi K, Hama C, Yoshioka T, Nabeshima Y. Cloning of the Drosophila prospero gene and its expression in ganglion mother cells. Biochem Biophys Res Commun. 1992 Feb 14;182(3):1326-32. PMID:1540176
- ↑ Xu C, Kauffmann RC, Zhang J, Kladny S, Carthew RW. Overlapping activators and repressors delimit transcriptional response to receptor tyrosine kinase signals in the Drosophila eye. Cell. 2000 Sep 29;103(1):87-97. PMID:11051550
- ↑ Ryter JM, Doe CQ, Matthews BW. Structure of the DNA binding region of prospero reveals a novel homeo-prospero domain. Structure. 2002 Nov;10(11):1541-9. PMID:12429095
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