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| - | [[Image:1d7m.gif|left|200px]] | |
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| + | ==COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I== |
| - | The line below this paragraph, containing "STRUCTURE_1d7m", creates the "Structure Box" on the page.
| + | <StructureSection load='1d7m' size='340' side='right'caption='[[1d7m]], [[Resolution|resolution]] 2.70Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1d7m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1D7M FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
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| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1d7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1d7m OCA], [https://pdbe.org/1d7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1d7m RCSB], [https://www.ebi.ac.uk/pdbsum/1d7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1d7m ProSAT]</span></td></tr> |
| - | {{STRUCTURE_1d7m| PDB=1d7m | SCENE= }}
| + | </table> |
| - | | + | == Function == |
| - | '''COILED-COIL DIMERIZATION DOMAIN FROM CORTEXILLIN I'''
| + | [https://www.uniprot.org/uniprot/CTXA_DICDI CTXA_DICDI] Acting-bundling protein. When linked to F-actin the actin filaments form preferentially anti-parallel bundles that associate into meshworks. Plays a major role in cytokinesis. Negatively regulates cortical localization of rapgap1.<ref>PMID:18039932</ref> |
| - | | + | == References == |
| - | | + | <references/> |
| - | ==Overview== | + | __TOC__ |
| - | BACKGROUND: The parallel two-stranded alpha-helical coiled coil is the most frequently encountered subunit-oligomerization motif in proteins. The simplicity and regularity of this motif have made it an attractive system to explore some of the fundamental principles of protein folding and stability and to test the principles of de novo design. RESULTS: The X-ray crystal structure of the 18-heptad-repeat alpha-helical coiled-coil domain of the actin-bundling protein cortexillin I from Dictyostelium discoideum is a tightly packed parallel two-stranded alpha-helical coiled coil. It harbors a distinct 14-residue sequence motif that is essential for coiled-coil formation, and is a prerequisite for the assembly of cortexillin I. The atomic structure reveals novel types of ionic coiled-coil interactions. In particular, the structure shows that a characteristic interhelical and intrahelical salt-bridge pattern, in combination with the hydrophobic interactions occurring at the dimer interface, is the key structural feature of its coiled-coil trigger site. CONCLUSIONS: The knowledge gained from the structure could be used in the de novo design of alpha-helical coiled coils for applications such as two-stage drug targeting and delivery systems, and in the design of coiled coils as templates for combinatorial helical libraries in drug discovery and as synthetic carrier molecules.
| + | </StructureSection> |
| - | | + | |
| - | ==About this Structure== | + | |
| - | 1D7M is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Dictyostelium_discoideum Dictyostelium discoideum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1D7M OCA].
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| - | | + | |
| - | ==Reference== | + | |
| - | The coiled-coil trigger site of the rod domain of cortexillin I unveils a distinct network of interhelical and intrahelical salt bridges., Burkhard P, Kammerer RA, Steinmetz MO, Bourenkov GP, Aebi U, Structure. 2000 Mar 15;8(3):223-30. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10745004 10745004]
| + | |
| | [[Category: Dictyostelium discoideum]] | | [[Category: Dictyostelium discoideum]] |
| - | [[Category: Single protein]] | + | [[Category: Large Structures]] |
| - | [[Category: Aebi, U.]] | + | [[Category: Aebi U]] |
| - | [[Category: Bourenkov, G P.]] | + | [[Category: Bourenkov GP]] |
| - | [[Category: Burkhard, P.]] | + | [[Category: Burkhard P]] |
| - | [[Category: Kammerer, R A.]] | + | [[Category: Kammerer RA]] |
| - | [[Category: Steinmetz, M O.]] | + | [[Category: Steinmetz MO]] |
| - | [[Category: Alpha helix]]
| + | |
| - | [[Category: Coiled-coil]]
| + | |
| - | [[Category: Coiled-coil trigger site]]
| + | |
| - | [[Category: Dimerization]]
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| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:32:11 2008''
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