1n3j

From Proteopedia

(Difference between revisions)

Current revision

Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1

Structural highlights

1n3j is a 2 chain structure with sequence from Paramecium bursaria Chlorella virus 1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

O41094_PBCV1

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Site-specific lysine methylation of histones by SET domains is a hallmark for epigenetic control of gene transcription in eukaryotic organisms. Here we report that a SET domain protein from Paramecium bursaria chlorella virus can specifically di-methylate Lys27 in histone H3, a modification implicated in gene silencing. The solution structure of the viral SET domain reveals a butterfly-shaped head-to-head symmetric dimer different from other known protein methyltransferases. Each subunit consists of a Greek-key antiparallel beta-barrel and a three-stranded open-faced sandwich that mediates the dimer interface. Cofactor S-adenosyl-L-methionine (SAM) binds at the opening of the beta-barrel, and amino acids C-terminal to Lys27 in H3 and in the flexible C-terminal tail of the enzyme confer the specificity of this viral histone methyltransferase.

A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3.,Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Manzur KL, Farooq A, Zeng L, Plotnikova O, Koch AW, Sachchidanand, Zhou MM. A dimeric viral SET domain methyltransferase specific to Lys27 of histone H3. Nat Struct Biol. 2003 Mar;10(3):187-96. PMID:12567185 doi:10.1038/nsb898

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1n3j"

@@ Line 1: / Line 1: @@
 ==Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1==
-<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''>
+<StructureSection load='1n3j' size='340' side='right'caption='[[1n3j]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chlorella_pbcv-1_virus Chlorella pbcv-1 virus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1n3j]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paramecium_bursaria_Chlorella_virus_1 Paramecium bursaria Chlorella virus 1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1N3J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1N3J FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A612L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10506 Chlorella PBCV-1 virus])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [http://pdbe.org/1n3j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [http://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1n3j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1n3j OCA], [https://pdbe.org/1n3j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1n3j RCSB], [https://www.ebi.ac.uk/pdbsum/1n3j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1n3j ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/O41094_PBCV1 O41094_PBCV1]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 33: / Line 35: @@
 __TOC__
 </StructureSection>
-[[Category: Chlorella pbcv-1 virus]]
 [[Category: Large Structures]]
-[[Category: Farooq, A]]
+[[Category: Paramecium bursaria Chlorella virus 1]]
-[[Category: Koch, A W]]
+[[Category: Farooq A]]
-[[Category: Manzur, K L]]
+[[Category: Koch AW]]
-[[Category: Plotnikova, O]]
+[[Category: Manzur KL]]
+[[Category: Plotnikova O]]
 [[Category: Sachchidanand]]
-[[Category: Zeng, L]]
+[[Category: Zeng L]]
-[[Category: Zhou, M M]]
+[[Category: Zhou M-M]]
-[[Category: Beta barrel]]
-[[Category: Homodimer]]
-[[Category: Transferase]]

1n3j

From Proteopedia

Current revision

Structure and Substrate of a Histone H3 Lysine Methyltransferase from Paramecium Bursaria Chlorella Virus 1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox