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| | ==The alternatively spliced PDZ2 domain of PTP-BL== | | ==The alternatively spliced PDZ2 domain of PTP-BL== |
| - | <StructureSection load='1ozi' size='340' side='right'caption='[[1ozi]], [[NMR_Ensembles_of_Models | 30 NMR models]]' scene=''> | + | <StructureSection load='1ozi' size='340' side='right'caption='[[1ozi]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ozi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1OZI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ozi]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OZI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OZI FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gm1|1gm1]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PTP-BL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ozi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozi OCA], [https://pdbe.org/1ozi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ozi RCSB], [https://www.ebi.ac.uk/pdbsum/1ozi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozi ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ozi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ozi OCA], [http://pdbe.org/1ozi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ozi RCSB], [http://www.ebi.ac.uk/pdbsum/1ozi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ozi ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PTN13_MOUSE PTN13_MOUSE]] Tyrosine phosphatase which regulates negatively FAS-induced apoptosis and NGFR-mediated pro-apoptotic signaling. | + | [https://www.uniprot.org/uniprot/PTN13_MOUSE PTN13_MOUSE] Tyrosine phosphatase which regulates negatively FAS-induced apoptosis and NGFR-mediated pro-apoptotic signaling. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Protein-tyrosine-phosphatase]]
| + | [[Category: Aelen J]] |
| - | [[Category: Aelen, J]] | + | [[Category: Hendriks W]] |
| - | [[Category: Berk, L van den]]
| + | [[Category: Oostendorp M]] |
| - | [[Category: Hendriks, W]] | + | [[Category: Vuister GW]] |
| - | [[Category: Oostendorp, M]] | + | [[Category: Walma T]] |
| - | [[Category: Vuister, G W]] | + | [[Category: Van den Berk L]] |
| - | [[Category: Walma, T]] | + | |
| - | [[Category: Adenomatous polyposis coli]] | + | |
| - | [[Category: All-beta protein]]
| + | |
| - | [[Category: Apc]]
| + | |
| - | [[Category: Hydrolase]]
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| - | [[Category: Interaction with c-termini]]
| + | |
| - | [[Category: Pdz domain]]
| + | |
| - | [[Category: Reversion induced lim]]
| + | |
| - | [[Category: Ril]]
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| Structural highlights
Function
PTN13_MOUSE Tyrosine phosphatase which regulates negatively FAS-induced apoptosis and NGFR-mediated pro-apoptotic signaling.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
PTP-BL is a large phosphatase that is implicated in cellular processes as diverse as cytokinesis, actin-cytoskeletal rearrangement, and apoptosis. Five PDZ domains mediate its cellular role by binding to the C termini of target proteins, forming multiprotein complexes. The second PDZ domain (PDZ2) binds to the C termini of the tumor suppressor protein APC and the LIM domain-containing protein RIL; however, in one splice variant, PDZ2as, a 5 residue insertion abrogates this binding. The insert causes distinct structural and dynamical changes in the alternatively spliced PDZ2: enlarging the L1 loop between beta2 and beta3, both lengthening and changing the orientation of the alpha2 helix, giving the base of the binding pocket less flexibility to accommodate ligands, and destabilizing the entire domain. These changes render the binding pocket incapable of binding C termini, possibly having implications in the functional role of PTP-BL.
A closed binding pocket and global destabilization modify the binding properties of an alternatively spliced form of the second PDZ domain of PTP-BL.,Walma T, Aelen J, Nabuurs SB, Oostendorp M, van den Berk L, Hendriks W, Vuister GW Structure. 2004 Jan;12(1):11-20. PMID:14725761[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Walma T, Aelen J, Nabuurs SB, Oostendorp M, van den Berk L, Hendriks W, Vuister GW. A closed binding pocket and global destabilization modify the binding properties of an alternatively spliced form of the second PDZ domain of PTP-BL. Structure. 2004 Jan;12(1):11-20. PMID:14725761
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