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| | <StructureSection load='1puj' size='340' side='right'caption='[[1puj]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='1puj' size='340' side='right'caption='[[1puj]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1puj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"vibrio_subtilis"_ehrenberg_1835 "vibrio subtilis" ehrenberg 1835]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1PUJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1puj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1PUJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YLQF ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Vibrio subtilis" Ehrenberg 1835])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1puj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1puj OCA], [http://pdbe.org/1puj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1puj RCSB], [http://www.ebi.ac.uk/pdbsum/1puj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1puj ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/1puj TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1puj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1puj OCA], [https://pdbe.org/1puj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1puj RCSB], [https://www.ebi.ac.uk/pdbsum/1puj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1puj ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1puj TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RBGA_BACSU RBGA_BACSU]] Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.<ref>PMID:16997968</ref> <ref>PMID:16431913</ref> <ref>PMID:16390447</ref> <ref>PMID:17613524</ref> | + | [https://www.uniprot.org/uniprot/RBGA_BACSU RBGA_BACSU] Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.<ref>PMID:16997968</ref> <ref>PMID:16431913</ref> <ref>PMID:16390447</ref> <ref>PMID:17613524</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Vibrio subtilis ehrenberg 1835]] | + | [[Category: Bacillus subtilis]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Buglino, J]] | + | [[Category: Buglino J]] |
| - | [[Category: Burley, S K]] | + | [[Category: Burley SK]] |
| - | [[Category: Kniewel, R]] | + | [[Category: Kniewel R]] |
| - | [[Category: Lima, C D]] | + | [[Category: Lima CD]] |
| - | [[Category: Structural genomic]]
| + | |
| - | [[Category: Gtpase]]
| + | |
| - | [[Category: NYSGXRC, New York SGX Research Center for Structural Genomics]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
RBGA_BACSU Essential protein that is required for a late step of 50S ribosomal subunit assembly. Has GTPase activity that is stimulated by interaction with the immature 50S ribosome subunit. Binds to the 23S rRNA. Required for the association of ribosomal proteins RplP and RpmA with the large subunit.[1] [2] [3] [4]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
References
- ↑ Schaefer L, Uicker WC, Wicker-Planquart C, Foucher AE, Jault JM, Britton RA. Multiple GTPases participate in the assembly of the large ribosomal subunit in Bacillus subtilis. J Bacteriol. 2006 Dec;188(23):8252-8. Epub 2006 Sep 22. PMID:16997968 doi:http://dx.doi.org/JB.01213-06
- ↑ Matsuo Y, Morimoto T, Kuwano M, Loh PC, Oshima T, Ogasawara N. The GTP-binding protein YlqF participates in the late step of 50 S ribosomal subunit assembly in Bacillus subtilis. J Biol Chem. 2006 Mar 24;281(12):8110-7. Epub 2006 Jan 23. PMID:16431913 doi:http://dx.doi.org/M512556200
- ↑ Uicker WC, Schaefer L, Britton RA. The essential GTPase RbgA (YlqF) is required for 50S ribosome assembly in Bacillus subtilis. Mol Microbiol. 2006 Jan;59(2):528-40. PMID:16390447 doi:http://dx.doi.org/MMI4948
- ↑ Matsuo Y, Oshima T, Loh PC, Morimoto T, Ogasawara N. Isolation and characterization of a dominant negative mutant of Bacillus subtilis GTP-binding protein, YlqF, essential for biogenesis and maintenance of the 50 S ribosomal subunit. J Biol Chem. 2007 Aug 31;282(35):25270-7. Epub 2007 Jul 5. PMID:17613524 doi:http://dx.doi.org/M703894200
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