1q45

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Current revision (08:12, 14 February 2024) (edit) (undo)
 
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<StructureSection load='1q45' size='340' side='right'caption='[[1q45]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='1q45' size='340' side='right'caption='[[1q45]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[1q45]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q45 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Q45 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[1q45]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q45 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Q45 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1q44|1q44]]</div></td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">At2g06050 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1q45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q45 OCA], [https://pdbe.org/1q45 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1q45 RCSB], [https://www.ebi.ac.uk/pdbsum/1q45 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1q45 ProSAT], [https://www.topsan.org/Proteins/CESG/1q45 TOPSAN]</span></td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/12-oxophytodienoate_reductase 12-oxophytodienoate reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.3.1.42 1.3.1.42] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1q45 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1q45 OCA], [http://pdbe.org/1q45 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1q45 RCSB], [http://www.ebi.ac.uk/pdbsum/1q45 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1q45 ProSAT], [http://www.topsan.org/Proteins/CESG/1q45 TOPSAN]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/OPR3_ARATH OPR3_ARATH]] Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program. In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).<ref>PMID:10973494</ref> <ref>PMID:19605548</ref>
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[https://www.uniprot.org/uniprot/OPR3_ARATH OPR3_ARATH] Specifically cleaves olefinic bonds in cyclic enones. Involved in the biosynthesis of jasmonic acid (JA) and perhaps in biosynthesis or metabolism of other oxylipin signaling moleclules. Required for the spatial and temporal regulation of JA levels during dehiscence of anthers, promoting the stomium degeneration program. In vitro, reduces 9S,13S-12-oxophytodienoic acid (9S,13S-OPDA) and 9R,13R-OPDA to 9S,13S-OPC-8:0 and 9R,13R-OPC-8:0, respectively. Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT).<ref>PMID:10973494</ref> <ref>PMID:19605548</ref>
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: 12-oxophytodienoate reductase]]
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[[Category: Arabidopsis thaliana]]
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[[Category: Arath]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Bingman, C A]]
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[[Category: Bingman CA]]
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[[Category: Structural genomic]]
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[[Category: Johnson KA]]
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[[Category: Johnson, K A]]
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[[Category: Phillips Jr GN]]
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[[Category: Phillips, G N]]
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[[Category: Smith DW]]
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[[Category: Smith, D W]]
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[[Category: Cesg]]
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[[Category: Flavoenzyme]]
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[[Category: Flavoprotein]]
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[[Category: Old Yellow Enzyme]]
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[[Category: Oxidoreductase]]
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[[Category: PSI, Protein structure initiative]]
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[[Category: Secondary messenger]]
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[[Category: Xenobiotic reductase]]
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Current revision

12-0xo-phytodienoate reductase isoform 3

PDB ID 1q45

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