7crb

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==Cryo-EM structure of plant NLR RPP1 LRR-ID domain in complex with ATR1==
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<StructureSection load='7crb' size='340' side='right'caption='[[7crb]]' scene=''>
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<StructureSection load='7crb' size='340' side='right'caption='[[7crb]], [[Resolution|resolution]] 3.16&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
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<table><tr><td colspan='2'>[[7crb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana] and [https://en.wikipedia.org/wiki/Hyaloperonospora_arabidopsidis_Emoy2 Hyaloperonospora arabidopsidis Emoy2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7CRB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7CRB FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7crb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crb OCA], [http://pdbe.org/7crb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7crb RCSB], [http://www.ebi.ac.uk/pdbsum/7crb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7crb ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.16&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7crb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7crb OCA], [https://pdbe.org/7crb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7crb RCSB], [https://www.ebi.ac.uk/pdbsum/7crb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7crb ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/ATR1_HYAAE ATR1_HYAAE] Secreted effector that acts as an elicitor of hypersensitive response (HR) specifically on plants carrying both defense protein RPP1 from several ecotypes including RPP1-NdA, RPP1-WsB, RPP1-EstA and RPP1-ZdrA.<ref>PMID:15894715</ref> <ref>PMID:22194907</ref> <ref>PMID:25671309</ref> <ref>PMID:26725254</ref> <ref>PMID:27427964</ref> <ref>PMID:29794019</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Direct or indirect recognition of pathogen-derived effectors by plant nucleotide-binding leucine-rich repeat (LRR) receptors (NLRs) initiates innate immune responses. The Hyaloperonospora arabidopsidis effector ATR1 activates the N-terminal Toll-interleukin-1 receptor (TIR) domain of Arabidopsis NLR RPP1. We report a cryo-electron microscopy structure of RPP1 bound by ATR1. The structure reveals a C-terminal jelly roll/Ig-like domain (C-JID) for specific ATR1 recognition. Biochemical and functional analyses show that ATR1 binds to the C-JID and the LRRs to induce an RPP1 tetrameric assembly required for nicotinamide adenine dinucleotide hydrolase (NADase) activity. RPP1 tetramerization creates two potential active sites, each formed by an asymmetric TIR homodimer. Our data define the mechanism of direct effector recognition by a plant NLR leading to formation of a signaling-active holoenzyme.
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Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme.,Ma S, Lapin D, Liu L, Sun Y, Song W, Zhang X, Logemann E, Yu D, Wang J, Jirschitzka J, Han Z, Schulze-Lefert P, Parker JE, Chai J Science. 2020 Dec 4;370(6521). pii: 370/6521/eabe3069. doi:, 10.1126/science.abe3069. PMID:33273071<ref>PMID:33273071</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 7crb" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Avirulence protein 3D structures|Avirulence protein 3D structures]]
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Arabidopsis thaliana]]
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[[Category: Hyaloperonospora arabidopsidis Emoy2]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Z-disk]]
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[[Category: Chai JJ]]
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[[Category: Han ZF]]
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[[Category: Jirschitzka J]]
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[[Category: Lapin D]]
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[[Category: Liu L]]
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[[Category: Logemann E]]
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[[Category: Ma SC]]
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[[Category: Parker JE]]
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[[Category: SchulzeLefert P]]
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[[Category: Song W]]
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[[Category: Sun Y]]
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[[Category: Wang J]]
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[[Category: Yu DL]]
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[[Category: Zhang XX]]

Current revision

Cryo-EM structure of plant NLR RPP1 LRR-ID domain in complex with ATR1

PDB ID 7crb

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