|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==NMR structure of the La motif of human La protein== | | ==NMR structure of the La motif of human La protein== |
| - | <StructureSection load='1s7a' size='340' side='right'caption='[[1s7a]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1s7a' size='340' side='right'caption='[[1s7a]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1s7a]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7A OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1S7A FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1s7a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S7A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S7A FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1s79|1s79]], [[1owx|1owx]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SSB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7a OCA], [https://pdbe.org/1s7a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s7a RCSB], [https://www.ebi.ac.uk/pdbsum/1s7a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7a ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1s7a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s7a OCA], [http://pdbe.org/1s7a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s7a RCSB], [http://www.ebi.ac.uk/pdbsum/1s7a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s7a ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LA_HUMAN LA_HUMAN]] Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.<ref>PMID:3192525</ref> <ref>PMID:2470590</ref> | + | [https://www.uniprot.org/uniprot/LA_HUMAN LA_HUMAN] Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.<ref>PMID:3192525</ref> <ref>PMID:2470590</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 33: |
Line 32: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alfano, C]] | + | [[Category: Alfano C]] |
| - | [[Category: Babon, J]] | + | [[Category: Babon J]] |
| - | [[Category: Conte, M R]] | + | [[Category: Conte MR]] |
| - | [[Category: Curry, S]] | + | [[Category: Curry S]] |
| - | [[Category: Jacks, A]] | + | [[Category: Jacks A]] |
| - | [[Category: Kelly, G]] | + | [[Category: Kelly G]] |
| - | [[Category: Sanfelice, D]] | + | [[Category: Sanfelice D]] |
| - | [[Category: Alpha/beta]]
| + | |
| - | [[Category: La motif]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| - | [[Category: Translation]]
| + | |
| - | [[Category: Winged helix domain]]
| + | |
| Structural highlights
Function
LA_HUMAN Binds to the 3' poly(U) terminii of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The La protein is a conserved component of eukaryotic ribonucleoprotein complexes that binds the 3' poly(U)-rich elements of nascent RNA polymerase III (pol III) transcripts to assist folding and maturation. This specific recognition is mediated by the N-terminal domain (NTD) of La, which comprises a La motif and an RNA recognition motif (RRM). We have determined the solution structures of both domains and show that the La motif adopts an alpha/beta fold that comprises a winged-helix motif elaborated by the insertion of three helices. Chemical shift mapping experiments show that these insertions are involved in RNA interactions. They further delineate a distinct surface patch on each domain-containing both basic and aromatic residues-that interacts with RNA and accounts for the cooperative binding of short oligonucleotides exhibited by the La NTD.
Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein.,Alfano C, Sanfelice D, Babon J, Kelly G, Jacks A, Curry S, Conte MR Nat Struct Mol Biol. 2004 Apr;11(4):323-9. Epub 2004 Mar 7. PMID:15004549[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chambers JC, Kenan D, Martin BJ, Keene JD. Genomic structure and amino acid sequence domains of the human La autoantigen. J Biol Chem. 1988 Dec 5;263(34):18043-51. PMID:3192525
- ↑ Gottlieb E, Steitz JA. Function of the mammalian La protein: evidence for its action in transcription termination by RNA polymerase III. EMBO J. 1989 Mar;8(3):851-61. PMID:2470590
- ↑ Alfano C, Sanfelice D, Babon J, Kelly G, Jacks A, Curry S, Conte MR. Structural analysis of cooperative RNA binding by the La motif and central RRM domain of human La protein. Nat Struct Mol Biol. 2004 Apr;11(4):323-9. Epub 2004 Mar 7. PMID:15004549 doi:http://dx.doi.org/10.1038/nsmb747
|
