1dcs

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[[Image:1dcs.gif|left|200px]]
 
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==DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS==
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The line below this paragraph, containing "STRUCTURE_1dcs", creates the "Structure Box" on the page.
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<StructureSection load='1dcs' size='340' side='right'caption='[[1dcs]], [[Resolution|resolution]] 1.30&Aring;' scene=''>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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== Structural highlights ==
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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<table><tr><td colspan='2'>[[1dcs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DCS FirstGlance]. <br>
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or leave the SCENE parameter empty for the default display.
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.3&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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{{STRUCTURE_1dcs| PDB=1dcs | SCENE= }}
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dcs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dcs OCA], [https://pdbe.org/1dcs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dcs RCSB], [https://www.ebi.ac.uk/pdbsum/1dcs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dcs ProSAT]</span></td></tr>
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</table>
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'''DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS'''
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== Function ==
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[https://www.uniprot.org/uniprot/CEFE_STRCL CEFE_STRCL] Catalyzes the step from penicillin N to deacetoxy-cephalosporin C.
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== Evolutionary Conservation ==
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==Overview==
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[[Image:Consurf_key_small.gif|200px|right]]
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Penicillins and cephalosporins are among the most widely used therapeutic agents. These antibiotics are produced from fermentation-derived materials as their chemical synthesis is not commercially viable. Unconventional steps in their biosynthesis are catalysed by Fe(II)-dependent oxidases/oxygenases; isopenicillin N synthase (IPNS) creates in one step the bicyclic nucleus of penicillins, and deacetoxycephalosporin C synthase (DAOCS) catalyses the expansion of the penicillin nucleus into the nucleus of cephalosporins. Both enzymes use dioxygen-derived ferryl intermediates in catalysis but, in contrast to IPNS, the ferryl form of DAOCS is produced by the oxidative splitting of a co-substrate, 2-oxoglutarate (alpha-ketoglutarate). This route of controlled ferryl formation and reaction is common to many mononuclear ferrous enzymes, which participate in a broader range of reactions than their well-characterized counterparts, the haem enzymes. Here we report the first crystal structure of a 2-oxoacid-dependent oxygenase. High-resolution structures for apo-DAOCS, the enzyme complexed with Fe(II), and with Fe(II) and 2-oxoglutarate, were obtained from merohedrally twinned crystals. Using a model based on these structures, we propose a mechanism for ferryl formation.
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Check<jmol>
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<jmolCheckbox>
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==About this Structure==
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/dc/1dcs_consurf.spt"</scriptWhenChecked>
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1DCS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_clavuligerus Streptomyces clavuligerus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DCS OCA].
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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<text>to colour the structure by Evolutionary Conservation</text>
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==Reference==
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</jmolCheckbox>
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Structure of a cephalosporin synthase., Valegard K, van Scheltinga AC, Lloyd MD, Hara T, Ramaswamy S, Perrakis A, Thompson A, Lee HJ, Baldwin JE, Schofield CJ, Hajdu J, Andersson I, Nature. 1998 Aug 20;394(6695):805-9. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9723623 9723623]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1dcs ConSurf].
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[[Category: Single protein]]
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<div style="clear:both"></div>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
[[Category: Streptomyces clavuligerus]]
[[Category: Streptomyces clavuligerus]]
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[[Category: Andersson, I.]]
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[[Category: Andersson I]]
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[[Category: Baldwin, J E.]]
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[[Category: Baldwin JE]]
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[[Category: Hajdu, J.]]
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[[Category: Hajdu J]]
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[[Category: Hara, T.]]
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[[Category: Hara T]]
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[[Category: Lee, H J.]]
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[[Category: Lee HJ]]
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[[Category: Lloyd, M D.]]
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[[Category: Lloyd MD]]
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[[Category: Perrakis, A.]]
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[[Category: Perrakis A]]
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[[Category: Ramaswamy, S.]]
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[[Category: Ramaswamy S]]
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[[Category: Scheltinga, A C.Terwisscha Van.]]
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[[Category: Schofield CJ]]
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[[Category: Schofield, C J.]]
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[[Category: Terwisscha Van Scheltinga AC]]
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[[Category: Thompson, A.]]
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[[Category: Thompson A]]
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[[Category: Valegard, K.]]
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[[Category: Valegard K]]
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[[Category: 2-oxoglutarate]]
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[[Category: Antibiotic biosynthesis]]
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[[Category: Antibiotic]]
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[[Category: Cephalosporin]]
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[[Category: Ferrous oxygenase]]
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[[Category: Merohedral twinning]]
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[[Category: Oxidoreductase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 13:42:12 2008''
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Current revision

DEACETOXYCEPHALOSPORIN C SYNTHASE FROM S. CLAVULIGERUS

PDB ID 1dcs

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