6zq6
From Proteopedia
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==Crystal structure of Chaetomium thermophilum Glycerol Kinase in complex with glycerol in P21212 space group== | ==Crystal structure of Chaetomium thermophilum Glycerol Kinase in complex with glycerol in P21212 space group== | ||
| - | <StructureSection load='6zq6' size='340' side='right'caption='[[6zq6]]' scene=''> | + | <StructureSection load='6zq6' size='340' side='right'caption='[[6zq6]], [[Resolution|resolution]] 2.30Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZQ6 OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6zq6]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ZQ6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ZQ6 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6zq6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6zq6 OCA], [https://pdbe.org/6zq6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6zq6 RCSB], [https://www.ebi.ac.uk/pdbsum/6zq6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6zq6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/G0SAG9_CHATD G0SAG9_CHATD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Glycerol is an organic compound that can be utilized as an alternative source of carbon by various organisms. One of the ways to assimilate glycerol by the cell is the phosphorylative catabolic pathway in which its activation is catalyzed by glycerol kinase (GK) and glycerol-3-phosphate (G3P) is formed. To date, several GK crystal structures from bacteria, archaea, and unicellular eukaryotic parasites have been solved. Herein, we present a series of crystal structures of GK from Chaetomium thermophilum (CtGK) in apo and glycerol-bound forms. In addition, we show the feasibility of an ADP-dependent glucokinase (ADPGK)-coupled enzymatic assay to measure the CtGK activity. New structures described in our work provide structural insights into the GK catalyzed reaction in the filamentous fungus and set the foundation for understanding the glycerol metabolism in eukaryotes. | ||
| + | |||
| + | Structural Characterization of Glycerol Kinase from the Thermophilic Fungus Chaetomium thermophilum.,Wilk P, Kuska K, Wator E, Malecki PH, Wos K, Tokarz P, Dubin G, Grudnik P Int J Mol Sci. 2020 Dec 16;21(24). pii: ijms21249570. doi: 10.3390/ijms21249570. PMID:33339113<ref>PMID:33339113</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6zq6" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Glycerol kinase|Glycerol kinase]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Grudnik P]] | [[Category: Grudnik P]] | ||
[[Category: Wator E]] | [[Category: Wator E]] | ||
[[Category: Wilk P]] | [[Category: Wilk P]] | ||
Current revision
Crystal structure of Chaetomium thermophilum Glycerol Kinase in complex with glycerol in P21212 space group
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