1gnt

From Proteopedia

(Difference between revisions)

Current revision

Hybrid Cluster Protein from Desulfovibrio vulgaris. X-ray structure at 1.25A resolution using synchrotron radiation.

Structural highlights

1gnt is a 1 chain structure with sequence from Desulfovibrio vulgaris. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HCP_NITV2 Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.[HAMAP-Rule:MF_00069]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The structures of the hybrid cluster proteins (HCPs) from the sulfate-reducing bacteria Desulfovibrio desulfuricans (ATCC 27774) and Desulfovibrio vulgaris (Hildenborough) have been elucidated at a resolution of 1.25 A using X-ray synchrotron radiation techniques. In the case of the D. desulfuricans protein, protein isolation, purification, crystallization and X-ray data collection were carried out under strict anaerobic conditions, whereas for the D. vulgaris protein the conditions were aerobic. However, both structures are essentially the same, comprising three domains and two iron-sulfur centres. One of these centres situated near the exterior of the molecules in domain 1 is a cubane [4Fe-4S] cluster, whereas the other, located at the interface of the three domains, contains the unusual four-iron cluster initially found in the D. vulgaris protein. Details of the structures and the associated EPR spectroscopy of the D. desulfuricans protein are reported herein. These structures show that the nature of the hybrid cluster, containing both oxygen and sulfur bridges, is independent of the presence of oxygen in the isolation and crystallization procedure and also does not vary significantly with changes in the oxidation state. The structures and amino acid sequences of the HCP are compared with the recently elucidated structure of the catalytic subunit of a carbon monoxide dehydrogenase from Carboxydothermus hydrogenoformans and related dehydrogenases. Electronic supplementary material to this paper can be obtained by using the Springer Link server located at http://dx.doi.org/10.1007/s00775-001-0326-y.

Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 A resolution using synchrotron radiation.,Macedo S, Mitchell EP, Romao CV, Cooper SJ, Coelho R, Liu MY, Xavier AV, LeGall J, Bailey S, Garner DC, Hagen WR, Teixeira M, Carrondo MA, Lindley P J Biol Inorg Chem. 2002 Apr;7(4-5):514-25. Epub 2002 Jan 23. PMID:11941509^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Macedo S, Mitchell EP, Romao CV, Cooper SJ, Coelho R, Liu MY, Xavier AV, LeGall J, Bailey S, Garner DC, Hagen WR, Teixeira M, Carrondo MA, Lindley P. Hybrid cluster proteins (HCPs) from Desulfovibrio desulfuricans ATCC 27774 and Desulfovibrio vulgaris (Hildenborough): X-ray structures at 1.25 A resolution using synchrotron radiation. J Biol Inorg Chem. 2002 Apr;7(4-5):514-25. Epub 2002 Jan 23. PMID:11941509 doi:10.1007/s00775-001-0326-y

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1gnt"

@@ Line 3: / Line 3: @@
 <StructureSection load='1gnt' size='340' side='right'caption='[[1gnt]], [[Resolution|resolution]] 1.25&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1gnt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29579 Atcc 29579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNT OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1GNT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1gnt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfovibrio_vulgaris Desulfovibrio vulgaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1GNT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1GNT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FSO:IRON/SULFUR/OXYGEN+HYBRID+CLUSTER'>FSO</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1e1d|1e1d]], [[1e2u|1e2u]], [[1e9v|1e9v]]</div></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gnt OCA], [https://pdbe.org/1gnt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1gnt RCSB], [https://www.ebi.ac.uk/pdbsum/1gnt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1gnt ProSAT]</span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1gnt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1gnt OCA], [http://pdbe.org/1gnt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1gnt RCSB], [http://www.ebi.ac.uk/pdbsum/1gnt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1gnt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HCP_DESVH HCP_DESVH]] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O (By similarity).
+[https://www.uniprot.org/uniprot/HCP_NITV2 HCP_NITV2] Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.[HAMAP-Rule:MF_00069]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 14: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gn/1gnt_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 33: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 29579]]
+[[Category: Desulfovibrio vulgaris]]
 [[Category: Large Structures]]
-[[Category: Bailey, S]]
+[[Category: Bailey S]]
-[[Category: Carrondo, M A]]
+[[Category: Carrondo MA]]
-[[Category: Coelho, R]]
+[[Category: Coelho R]]
-[[Category: Cooper, S J]]
+[[Category: Cooper SJ]]
-[[Category: Garner, D C]]
+[[Category: Garner DC]]
-[[Category: Hagen, W R]]
+[[Category: Hagen WR]]
-[[Category: Legall, J]]
+[[Category: Legall J]]
-[[Category: Lindley, P]]
+[[Category: Lindley P]]
-[[Category: Liu, M Y]]
+[[Category: Liu MY]]
-[[Category: Macedo, S]]
+[[Category: Macedo S]]
-[[Category: Mitchell, E P]]
+[[Category: Mitchell EP]]
-[[Category: Romao, C V]]
+[[Category: Romao CV]]
-[[Category: Teixeira, M]]
+[[Category: Teixeira M]]
-[[Category: Xavier, A V]]
+[[Category: Xavier AV]]
-[[Category: Aerobic desulfovibrio vulgari]]
-[[Category: Hybrid cluster protein]]
-[[Category: Oxidoreductase]]

1gnt

From Proteopedia

Current revision

Hybrid Cluster Protein from Desulfovibrio vulgaris. X-ray structure at 1.25A resolution using synchrotron radiation.

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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