1tr8

From Proteopedia

(Difference between revisions)

Current revision

Crystal Structure of archaeal Nascent Polypeptide-associated Complex (aeNAC)

Structural highlights

1tr8 is a 2 chain structure with sequence from Methanothermobacter marburgensis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.27Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Nascent polypeptide-associated complex (NAC) was identified in eukaryotes as the first cytosolic factor that contacts the nascent polypeptide chain emerging from the ribosome. NAC is highly conserved from yeast to humans. Mutations in NAC cause severe embryonically lethal phenotypes in mice, Drosophila, and Caenorhabditis elegans. NAC was suggested to protect the nascent chain from inappropriate early interactions with cytosolic factors. Eukaryotic NAC is a heterodimer with two subunits sharing substantial homology with each other. All sequenced archaebacterial genomes exhibit only one gene homologous to the NAC subunits. Here we present the first archaebacterial NAC homolog. It forms a homodimer, and as eukaryotic NAC it is associated with ribosomes and contacts the emerging nascent chain on the ribosome. We present the first crystal structure of a NAC protein revealing two structural features: (i) a novel unique protein fold that mediates dimerization of the complex, and (ii) a ubiquitin-associated domain that suggests a yet unidentified role for NAC in the cellular protein quality control system via the ubiquitination pathway. Based on the presented structure we propose a model for the eukaryotic heterodimeric NAC domain.

The crystal structure of archaeal nascent polypeptide-associated complex (NAC) reveals a unique fold and the presence of a ubiquitin-associated domain.,Spreter T, Pech M, Beatrix B J Biol Chem. 2005 Apr 22;280(16):15849-54. Epub 2005 Jan 22. PMID:15665334^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Spreter T, Pech M, Beatrix B. The crystal structure of archaeal nascent polypeptide-associated complex (NAC) reveals a unique fold and the presence of a ubiquitin-associated domain. J Biol Chem. 2005 Apr 22;280(16):15849-54. Epub 2005 Jan 22. PMID:15665334 doi:10.1074/jbc.M500160200

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1tr8"

Categories: Large Structures | Methanothermobacter marburgensis | Beatrix B | Pech M | Spreter T

@@ Line 3: / Line 3: @@
 <StructureSection load='1tr8' size='340' side='right'caption='[[1tr8]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1tr8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Dsm_2133 Dsm 2133]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TR8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TR8 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1tr8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_marburgensis Methanothermobacter marburgensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TR8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TR8 FirstGlance]. <br>
-</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">MTH177 (amonoacids 19-117) ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145263 DSM 2133])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1tr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tr8 OCA], [http://pdbe.org/1tr8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tr8 RCSB], [http://www.ebi.ac.uk/pdbsum/1tr8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tr8 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tr8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tr8 OCA], [https://pdbe.org/1tr8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tr8 RCSB], [https://www.ebi.ac.uk/pdbsum/1tr8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tr8 ProSAT]</span></td></tr>
 </table>
-== Function ==
-[[http://www.uniprot.org/uniprot/NAC_METTM NAC_METTM]] Contacts the emerging nascent chain on the ribosome.[HAMAP-Rule:MF_00814]
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 13: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tr/1tr8_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 33: / Line 31: @@
 __TOC__
 </StructureSection>
-[[Category: Dsm 2133]]
 [[Category: Large Structures]]
-[[Category: Beatrix, B]]
+[[Category: Methanothermobacter marburgensis]]
-[[Category: Pech, M]]
+[[Category: Beatrix B]]
-[[Category: Spreter, T]]
+[[Category: Pech M]]
-[[Category: Chaperone]]
+[[Category: Spreter T]]
-[[Category: Nascent polypeptide-associated complex]]
-[[Category: Ribosome]]
-[[Category: Uba-domain]]
-[[Category: Ubiquitin]]

1tr8

From Proteopedia

Current revision

Crystal Structure of archaeal Nascent Polypeptide-associated Complex (aeNAC)

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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