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| | <StructureSection load='6uso' size='340' side='right'caption='[[6uso]], [[Resolution|resolution]] 2.54Å' scene=''> | | <StructureSection load='6uso' size='340' side='right'caption='[[6uso]], [[Resolution|resolution]] 2.54Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6uso]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Red_flour_beetle Red flour beetle]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6USO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6USO FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6uso]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Tribolium_castaneum Tribolium castaneum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6USO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6USO FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.54Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TERT, TcasGA2_TC010963 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7070 Red flour beetle])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/RNA-directed_DNA_polymerase RNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.49 2.7.7.49] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uso OCA], [https://pdbe.org/6uso PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uso RCSB], [https://www.ebi.ac.uk/pdbsum/6uso PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uso ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uso FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uso OCA], [http://pdbe.org/6uso PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uso RCSB], [http://www.ebi.ac.uk/pdbsum/6uso PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uso ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q0QHL8_TRICA Q0QHL8_TRICA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | | | |
| | ==See Also== | | ==See Also== |
| - | *[[Telomerase|Telomerase]] | + | *[[Telomerase 3D structures|Telomerase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: RNA-directed DNA polymerase]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Red flour beetle]] | + | [[Category: Tribolium castaneum]] |
| - | [[Category: Freudenthal, B D]] | + | [[Category: Freudenthal BD]] |
| - | [[Category: Schaich, M A]] | + | [[Category: Schaich MA]] |
| - | [[Category: Nuclear protein]]
| + | |
| - | [[Category: Polymerase]]
| + | |
| - | [[Category: Reverse transcriptase]]
| + | |
| - | [[Category: Telomerase]]
| + | |
| - | [[Category: Transferase-rna-dna complex]]
| + | |
| Structural highlights
Function
Q0QHL8_TRICA
Publication Abstract from PubMed
Telomerase extends telomere sequences at chromosomal ends to protect genomic DNA. During this process it must select the correct nucleotide from a pool of nucleotides with various sugars and base pairing properties, which is critically important for the proper capping of telomeric sequences by shelterin. Unfortunately, how telomerase selects correct nucleotides is unknown. Here, we determined structures of Tribolium castaneum telomerase reverse transcriptase (TERT) throughout its catalytic cycle and mapped the active site residues responsible for nucleoside selection, metal coordination, triphosphate binding, and RNA template stabilization. We found that TERT inserts a mismatch or ribonucleotide ~1 in 10,000 and ~1 in 14,000 insertion events, respectively. At biological ribonucleotide concentrations, these rates translate to ~40 ribonucleotides inserted per 10 kilobases. Human telomerase assays determined a conserved tyrosine steric gate regulates ribonucleotide insertion into telomeres. Cumulatively, our work provides insight into how telomerase selects the proper nucleotide to maintain telomere integrity.
Mechanisms of nucleotide selection by telomerase.,Schaich MA, Sanford SL, Welfer GA, Johnson SA, Khoang TH, Opresko PL, Freudenthal BD Elife. 2020 Jun 5;9. pii: 55438. doi: 10.7554/eLife.55438. PMID:32501800[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Schaich MA, Sanford SL, Welfer GA, Johnson SA, Khoang TH, Opresko PL, Freudenthal BD. Mechanisms of nucleotide selection by telomerase. Elife. 2020 Jun 5;9. pii: 55438. doi: 10.7554/eLife.55438. PMID:32501800 doi:http://dx.doi.org/10.7554/eLife.55438
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