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| | ==Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain== | | ==Solution structure of a mouse cytoplasmic Roadblock/LC7 dynein light chain== |
| - | <StructureSection load='1y4o' size='340' side='right'caption='[[1y4o]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1y4o' size='340' side='right'caption='[[1y4o]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1y4o]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Y4O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1y4o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Y4O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Y4O FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dncl2a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4o OCA], [http://pdbe.org/1y4o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1y4o RCSB], [http://www.ebi.ac.uk/pdbsum/1y4o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4o ProSAT], [http://www.topsan.org/Proteins/CESG/1y4o TOPSAN]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1y4o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1y4o OCA], [https://pdbe.org/1y4o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1y4o RCSB], [https://www.ebi.ac.uk/pdbsum/1y4o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1y4o ProSAT], [https://www.topsan.org/Proteins/CESG/1y4o TOPSAN]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/DLRB1_MOUSE DLRB1_MOUSE]] Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. | + | [https://www.uniprot.org/uniprot/DLRB1_MOUSE DLRB1_MOUSE] Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Structural genomic]]
| + | [[Category: Lee MS]] |
| - | [[Category: Lee, M S]] | + | [[Category: Markley JL]] |
| - | [[Category: Markley, J L]] | + | [[Category: Song J]] |
| - | [[Category: Song, J]] | + | [[Category: Tyler EM]] |
| - | [[Category: Tyler, E M]] | + | [[Category: Tyler RC]] |
| - | [[Category: Tyler, R C]] | + | |
| - | [[Category: Cesg]]
| + | |
| - | [[Category: Contractile protein]]
| + | |
| - | [[Category: Dynein light chain]]
| + | |
| - | [[Category: PSI, Protein structure initiative]]
| + | |
| Structural highlights
Function
DLRB1_MOUSE Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Roadblock/LC7 is a member of a class of dynein light chains involved in regulating the function of the dynein complex. We have determined the three-dimensional structure of isoform 1 of the mouse Roadblock/LC7 cytoplasmic dynein light chain (robl1_mouse) by NMR spectroscopy. In contrast to a previously reported NMR structure of the human homolog with 96% sequence identity (PDB 1TGQ), which showed the protein as a monomer, our results indicate clearly that robl1 exists as a symmetric homodimer. The two beta3-strands pair with each other and form a continuous ten-stranded beta-sheet. The 25-residue alpha2-helix from one subunit packs antiparallel to that of the other subunit on the face of the beta-sheet. Zipper-like hydrophobic contacts between the two helices serve to stabilize the dimer. Through an NMR titration experiment, we localized the site on robl1_mouse that interacts with the 40 residue peptide spanning residues 243 through 282 of IC74-1_rat. These results provide physical evidence for a symmetrical interaction between dimeric robl1 and the two molecules of IC74-1 in the dynein complex.
Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex.,Song J, Tyler RC, Lee MS, Tyler EM, Markley JL J Mol Biol. 2005 Dec 16;354(5):1043-51. Epub 2005 Nov 2. PMID:16289575[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Song J, Tyler RC, Lee MS, Tyler EM, Markley JL. Solution structure of isoform 1 of Roadblock/LC7, a light chain in the dynein complex. J Mol Biol. 2005 Dec 16;354(5):1043-51. Epub 2005 Nov 2. PMID:16289575 doi:10.1016/j.jmb.2005.10.017
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