6yhw

From Proteopedia

(Difference between revisions)

Current revision

Co-crystals in the P212121 space group, of a beta-cyclodextrin spacered by triazole heptyl from alpha-D-mannose, with FimH lectin at 2.00 A resolution.

Structural highlights

6yhw is a 2 chain structure with sequence from Escherichia coli. This structure supersedes the now removed PDB entry 5ab1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.962Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

FIMH_ECOLI Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin responsible for the binding to D-mannose. It is laterally positioned at intervals in the structure of the type 1 fimbriae. In order to integrate FimH in the fimbriae FimF and FimG are needed.

Publication Abstract from PubMed

Recently, we extended the anti-adhesive concept showing that potent FimH antagonists can block the attachment of adherent-invasive E. coli (AIEC) colonizing the intestinal mucosa of patients with Crohn's disease (CD). In this work, we designed a small library of analogs of heptylmannoside (HM), a previously identified nanomolar FimH inhibitor, but displaying poor in vivo anti-adhesive effects. The anomeric oxygen atom was replaced by a sulfur or a methylene group to prevent hydrolysis by intestinal glycosidases, and chemical groups were attached at the end of the alkyl tail. Importantly, a lead compound was shown to reduce AIEC levels in the feces, colonic and ileal mucosa after oral administration in a transgenic mouse model of CD. The compound showed a preferable low bioavailability suggesting the possibility of setting up an innovative antiadhesive therapy for CD patients in which AIEC play a key role, with the water-soluble and non-cytotoxic FimH, antagonists developed here.

The anti-adhesive strategy in Crohn's disease: orally active mannosides to decolonize pathogenic Escherichia coli from the gut.,Dorta DA, Sivignon A, Chalopin T, Dumych T, Roos G, Bilyy R, Deniaud D, Krammer EM, De Ruyck J, Lensink M, Bouckaert J, Barnich N, Gouin SG Chembiochem. 2016 Mar 4. doi: 10.1002/cbic.201600018. PMID:26946458^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Dorta DA, Sivignon A, Chalopin T, Dumych T, Roos G, Bilyy R, Deniaud D, Krammer EM, De Ruyck J, Lensink M, Bouckaert J, Barnich N, Gouin SG. The anti-adhesive strategy in Crohn's disease: orally active mannosides to decolonize pathogenic Escherichia coli from the gut. Chembiochem. 2016 Mar 4. doi: 10.1002/cbic.201600018. PMID:26946458 doi:http://dx.doi.org/10.1002/cbic.201600018

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6yhw"

Categories: Escherichia coli | Large Structures | Bouckaert J | De Ruyck J

@@ Line 3: / Line 3: @@
 <StructureSection load='6yhw' size='340' side='right'caption='[[6yhw]], [[Resolution|resolution]] 1.96&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6yhw]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5ab1 5ab1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHW OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YHW FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6yhw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5ab1 5ab1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YHW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YHW FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=HP6:HEPTANE'>HP6</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=TA5:2H-1,2,3-TRIAZOL-4-YLMETHANOL'>TA5</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.962&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">fimH, fimH_1, fimH_2, fimH_3, sfaH_2, A6581_19835, ACN81_00940, AKG99_10920, AM446_24165, AM464_16260, AUQ13_11280, AWG78_008695, B6V57_25500, B9M99_24715, B9T59_08965, BON65_08035, BON69_13145, BON76_10045, BON87_19590, BvCms12BK_05011, BvCms2454_04206, C2U48_10910, C5P44_07835, C6B13_05760, CR538_22045, CRX46_08755, D2188_19245, D9G11_20470, D9H70_11375, D9I97_23340, D9J60_03345, DJ503_16470, DNQ45_27015, DS732_03965, DU321_25405, DXT73_23425, E0L12_19135, EC3234A_192c00060, ECONIH1_25860, ECTO6_04155, EIA21_01475, ELV08_23920, EQ830_16720, ERS085383_04267, F0312_06085, F1E19_12715, FNJ69_21090, FNJ83_00985, FV293_11400, FY127_19655, MS6198_50840, MS8345_04977, NCTC10090_02366, NCTC8450_00821, NCTC9045_05036, SAMEA3472056_01232, YDC107_3079 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=HP6:HEPTANE'>HP6</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=PRD_900012:beta-cyclodextrin'>PRD_900012</scene>, <scene name='pdbligand=TA5:2H-1,2,3-TRIAZOL-4-YLMETHANOL'>TA5</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhw OCA], [http://pdbe.org/6yhw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yhw RCSB], [http://www.ebi.ac.uk/pdbsum/6yhw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhw ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yhw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yhw OCA], [https://pdbe.org/6yhw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yhw RCSB], [https://www.ebi.ac.uk/pdbsum/6yhw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yhw ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/FIMH_ECOLI FIMH_ECOLI] Involved in regulation of length and mediation of adhesion of type 1 fimbriae (but not necessary for the production of fimbriae). Adhesin responsible for the binding to D-mannose. It is laterally positioned at intervals in the structure of the type 1 fimbriae. In order to integrate FimH in the fimbriae FimF and FimG are needed.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 24: / Line 26: @@
 __TOC__
 </StructureSection>
-[[Category: Bacillus coli migula 1895]]
+[[Category: Escherichia coli]]
 [[Category: Large Structures]]
-[[Category: Bouckaert, J]]
+[[Category: Bouckaert J]]
-[[Category: Ruyck, J de]]
+[[Category: De Ruyck J]]
-[[Category: Beta-cyclodextrin host-guest interactions inhibitor design]]
-[[Category: Cell adhesion]]

6yhw

From Proteopedia

Current revision

Co-crystals in the P212121 space group, of a beta-cyclodextrin spacered by triazole heptyl from alpha-D-mannose, with FimH lectin at 2.00 A resolution.

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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