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| | <StructureSection load='7byk' size='340' side='right'caption='[[7byk]], [[Resolution|resolution]] 2.65Å' scene=''> | | <StructureSection load='7byk' size='340' side='right'caption='[[7byk]], [[Resolution|resolution]] 2.65Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7byk]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33152 Atcc 33152]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=7BYK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7byk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BYK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BYK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DI026_09185 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=446 ATCC 33152])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=7byk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7byk OCA], [http://pdbe.org/7byk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=7byk RCSB], [http://www.ebi.ac.uk/pdbsum/7byk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=7byk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7byk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7byk OCA], [https://pdbe.org/7byk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7byk RCSB], [https://www.ebi.ac.uk/pdbsum/7byk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7byk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q5ZU83_LEGPH Q5ZU83_LEGPH] |
| - | Legionella pneumophila infects alveolar macrophages and can cause life-threatening pneumonia in humans. Upon internalization into the host cell, L. pneumophila injects numerous effector proteins into the host cytoplasm as a part of its pathogenesis. LegK7 is an effector kinase of L. pneumophila that functionally mimics the eukaryotic Mst kinase and phosphorylates the host MOB1 protein to exploit the Hippo pathway. To elucidate the LegK7 activation mechanism, we determined the apo structure of LegK7 in an inactive form and performed a comparative analysis of LegK7 structures. LegK7 is a non-RD kinase that contains an activation segment that is ordered, irrespective of stimulation, through a unique beta-hairpin-containing segment, and it does not require phosphorylation of the activation segment for activation. Instead, bacterial LegK7 becomes an active kinase via its heterologous molecular interaction with the host MOB1 protein. MOB1 binding triggers reorientation of the two lobes of the kinase domain, as well as a structural change in the interlobe hinge region in LegK7, consequently reshaping the LegK7 structure into an ATP binding-compatible closed conformation. Furthermore, we reveal that LegK7 is an atypical kinase that contains an N-terminal capping domain and a hydrophilic interlobe linker motif, which play key roles in the MOB1-induced activation of LegK7.
| + | |
| - | | + | |
| - | Activation of the Legionella pneumophila LegK7 effector kinase by the host MOB1 protein.,Cheol Park S, Yeon Cho S, Hee Kim T, Yeol Ko K, Seok Song W, Goo Kang S, Lee GS, Yoon SI J Mol Biol. 2020 Dec 9:166746. doi: 10.1016/j.jmb.2020.166746. PMID:33309852<ref>PMID:33309852</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 7byk" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 33152]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kim, T H]] | |
| - | [[Category: Park, S C]] | |
| - | [[Category: Yoon, S I]] | |
| | [[Category: Legionella pneumophila]] | | [[Category: Legionella pneumophila]] |
| - | [[Category: Protein kinase]] | + | [[Category: Kim TH]] |
| - | [[Category: Transferase]] | + | [[Category: Park SC]] |
| | + | [[Category: Yoon SI]] |
