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| | <StructureSection load='1z6r' size='340' side='right'caption='[[1z6r]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='1z6r' size='340' side='right'caption='[[1z6r]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1z6r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z6R OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Z6R FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1z6r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z6R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z6R FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mlc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z6r OCA], [https://pdbe.org/1z6r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z6r RCSB], [https://www.ebi.ac.uk/pdbsum/1z6r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z6r ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1z6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z6r OCA], [http://pdbe.org/1z6r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z6r RCSB], [http://www.ebi.ac.uk/pdbsum/1z6r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z6r ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MLC_ECOLI MLC_ECOLI]] Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.<ref>PMID:10464268</ref> <ref>PMID:11361067</ref> | + | [https://www.uniprot.org/uniprot/MLC_ECOLI MLC_ECOLI] Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.<ref>PMID:10464268</ref> <ref>PMID:11361067</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus coli migula 1895]] | + | [[Category: Escherichia coli]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Boos, W]] | + | [[Category: Boos W]] |
| - | [[Category: Diederichs, K]] | + | [[Category: Diederichs K]] |
| - | [[Category: Gerber, K]] | + | [[Category: Gerber K]] |
| - | [[Category: Schiefner, A]] | + | [[Category: Schiefner A]] |
| - | [[Category: Seitz, S]] | + | [[Category: Seitz S]] |
| - | [[Category: Welte, W]] | + | [[Category: Welte W]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Helix-turn-helix]]
| + | |
| - | [[Category: Metalloprotein]]
| + | |
| - | [[Category: Phosphotransferase system]]
| + | |
| - | [[Category: Rok family protein]]
| + | |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcriptional repressor]]
| + | |
| Structural highlights
Function
MLC_ECOLI Transcriptional repressor that regulates the expression of proteins that are part of the phosphotransferase system for sugar uptake. Regulates the expression of malT.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Mlc from Escherichia coli is a transcriptional repressor controlling the expression of a number of genes encoding enzymes of the phosphotransferase system (PTS), including ptsG and manXYZ, the specific enzyme II for glucose and mannose PTS transporters. In addition, Mlc controls the transcription of malT, the gene of the global activator of the mal regulon. The inactivation of Mlc as a repressor is mediated by binding to an actively transporting PtsG (EIICB(Glc)). Here we report the crystal structure of Mlc at 2.7 A resolution representing the first described structure of an ROK (repressors, open reading frames, and kinases) family protein. Mlc forms stable dimers thus explaining its binding affinity to palindromic operator sites. The N-terminal helix-turn-helix domain of Mlc is stabilized by the amphipathic C-terminal helix implicated earlier in EIICB(Glc) binding. Furthermore, the structure revealed a metal-binding site within the cysteine-rich ROK consensus motif that coordinates a structurally important zinc ion. A strongly reduced repressor activity was observed when two of the zinc-coordinating cysteine residues were exchanged against serine or alanine, demonstrating the role of zinc in Mlc-mediated repressor function. The structures of a putative fructokinase from Bacillus subtilis, the glucokinase from Escherichia coli, and a glucomannokinase from Arthrobacter sp. showed high structural homology to the ROK family part of Mlc.
The crystal structure of Mlc, a global regulator of sugar metabolism in Escherichia coli.,Schiefner A, Gerber K, Seitz S, Welte W, Diederichs K, Boos W J Biol Chem. 2005 Aug 12;280(32):29073-9. Epub 2005 Jun 1. PMID:15929984[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kim SY, Nam TW, Shin D, Koo BM, Seok YJ, Ryu S. Purification of Mlc and analysis of its effects on the pts expression in Escherichia coli. J Biol Chem. 1999 Sep 3;274(36):25398-402. PMID:10464268
- ↑ Plumbridge J. Regulation of PTS gene expression by the homologous transcriptional regulators, Mlc and NagC, in Escherichia coli (or how two similar repressors can behave differently). J Mol Microbiol Biotechnol. 2001 Jul;3(3):371-80. PMID:11361067
- ↑ Schiefner A, Gerber K, Seitz S, Welte W, Diederichs K, Boos W. The crystal structure of Mlc, a global regulator of sugar metabolism in Escherichia coli. J Biol Chem. 2005 Aug 12;280(32):29073-9. Epub 2005 Jun 1. PMID:15929984 doi:10.1074/jbc.M504215200
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