Replication protein E1

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<StructureSection load='1ksx' size='340' side='right' caption='Bovine papillomavirus 1 replication protein E1 complex with DNA (PDB code [[1ksx]])' scene=''>
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<StructureSection load='1ksx' size='340' side='right' caption='Bovine papillomavirus 1 replication protein E1 complex with DNA (PDB code [[1ksx]])' scene='87/873777/Cv/1'>
==Function==
==Function==
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'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. RPE2 is found also in bovine papillomavirus (BPV).
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'''Replication protein E1''' (RPE1) is a human papillomavirus (HPV) essential protein which is required for its replication. RPE1 recognizes the HPV origin of replication (ori) with the aid of '''regulatory protein E2''' (RPE2). The resultant helicase complex initiates DNA unwinding to provide the template of progeny DNA synthesis, The HPV ori contains one RPE1 binding site and 2 RPE2 binding sites<ref>PMID:12086149</ref>. RPE1 is found also in bovine papillomavirus (BPV).
== Disease ==
== Disease ==
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RPE2 induces apoptosis of its host cells<ref>PMID:26906543</ref>.
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RPE1 has been shown to mitigate host cell defenses leading to the development of cancer induces apoptosis of its host cells<ref>PMID:29495005</ref>.
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==Relevance==
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The detection of RPE1 mRNA and promoter methylation may serve as cancer prognostic marker.
== Structural highlights ==
== Structural highlights ==
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The complex between HPV6a DBD and 18-bp DNA (PDB code [[2ayg]]) shows the binding of 2 recognition helices of the dimer to 2 successive major grooves of the target DNA making numerous contacts to the DNA backbone phosphate atoms and to its bases<ref>PMID:16914454</ref>.
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The complex between BPV1 DBD and 21-bp DNA (PDB code [[1ksx]]) shows 2 DBD-DNA binding modules: a <scene name='87/873777/Cv/2'>binding loop</scene> and a <scene name='87/873777/Cv/4'>binding helix</scene> each interacting with a single strand. The protein-DNA interactions are hydrophilic to phosphate atoms and van der Waals hydrophobic ones<ref>PMID:11889054</ref>.
</StructureSection>
</StructureSection>
==3D structures of replication protein E1==
==3D structures of replication protein E1==
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Domains: helicase 301-605; DNA-binding (DBD) 286-365
Domains: helicase 301-605; DNA-binding (DBD) 286-365
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[[1r9w]] - RPE1 DBD - HPV18<br />
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[[1r9w]] - HpvRPE1 DBD – Human papilloma virus 18<br />
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[[1tue]] - RPE1 428-631 (mutant) + RPE2 TAD<br />
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[[1tue]] - HpvRPE1 428-631 (mutant) + RPE2 TAD<br />
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[[2v9p]] - RPE1 helicase domain - BPV1<br />
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[[2gxa]] - DpvRPE1 helicase domain + DNA + ADP – Delta papilloma virus 4<br />
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[[5a9k]] - RPE1 helicase domain - Cryo EM<br />
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[[5a9k]] - BpvRPE1 helicase domain – Bovine papilloma virus - Cryo EM<br />
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[[2gxa]] - RPE1 helicase domain + DNA + ADP<br />
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[[2v9p]] - BpvRPE1 helicase domain <br />
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[[1f08]] - RPE1 DBD<br />
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[[1f08]] - BpvRPE1 DBD<br />
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[[1ksx]], [[1ksy]] - RPE1 DBD + DNA<br />
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[[1ksx]], [[1ksy]] - BpvRPE1 DBD + DNA<br />
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[[7apd]] - BpvRPE1 helicase domain + DNA – Cryo EM<br />
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Bovine papillomavirus 1 replication protein E1 complex with DNA (PDB code 1ksx)

Drag the structure with the mouse to rotate

3D structures of replication protein E1

Updated on 13-February-2022

Domains: helicase 301-605; DNA-binding (DBD) 286-365

1r9w - HpvRPE1 DBD – Human papilloma virus 18
1tue - HpvRPE1 428-631 (mutant) + RPE2 TAD
2gxa - DpvRPE1 helicase domain + DNA + ADP – Delta papilloma virus 4
5a9k - BpvRPE1 helicase domain – Bovine papilloma virus - Cryo EM
2v9p - BpvRPE1 helicase domain
1f08 - BpvRPE1 DBD
1ksx, 1ksy - BpvRPE1 DBD + DNA
7apd - BpvRPE1 helicase domain + DNA – Cryo EM

References

  1. ↑ Wilson VG, West M, Woytek K, Rangasamy D. Papillomavirus E1 proteins: form, function, and features. Virus Genes. 2002 Jun;24(3):275-90. doi: 10.1023/a:1015336817836. PMID:12086149 doi:http://dx.doi.org/10.1023/a:1015336817836
  2. ↑ Baedyananda F, Chaiwongkot A, Bhattarakosol P. Elevated HPV16 E1 Expression Is Associated with Cervical Cancer Progression. Intervirology. 2017;60(5):171-180. doi: 10.1159/000487048. Epub 2018 Mar 1. PMID:29495005 doi:http://dx.doi.org/10.1159/000487048
  3. ↑ Enemark EJ, Stenlund A, Joshua-Tor L. Crystal structures of two intermediates in the assembly of the papillomavirus replication initiation complex. EMBO J. 2002 Mar 15;21(6):1487-96. PMID:11889054 doi:http://dx.doi.org/10.1093/emboj/21.6.1487

Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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