7do5
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase(apo-form)== | ==Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase(apo-form)== | ||
| - | <StructureSection load='7do5' size='340' side='right'caption='[[7do5]]' scene=''> | + | <StructureSection load='7do5' size='340' side='right'caption='[[7do5]], [[Resolution|resolution]] 1.84Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DO5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7do5]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Azotobacter_vinelandii_DJ Azotobacter vinelandii DJ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DO5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DO5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7do5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7do5 OCA], [https://pdbe.org/7do5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7do5 RCSB], [https://www.ebi.ac.uk/pdbsum/7do5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7do5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.836Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7do5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7do5 OCA], [https://pdbe.org/7do5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7do5 RCSB], [https://www.ebi.ac.uk/pdbsum/7do5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7do5 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/C1DMX5_AZOVD C1DMX5_AZOVD] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Several microorganisms can utilize L-rhamnose as a carbon and energy source through the non-phosphorylative metabolic pathway, in which L-rhamnose 1-dehydrogenase (RhaDH) catalyzes the NAD(P)(+) -dependent oxidization of L-rhamnose to L-rhamnono-1,4-lactone. We herein investigated the crystal structures of RhaDH from Azotobacter vinelandii in ligand-free, NAD(+) -bound, NADP(+) -bound, and L-rhamnose- and NAD(+) -bound forms at 1.9, 2.1, 2.4, and 1.6 A resolution, respectively. The significant interactions with the 2'-phosphate group of NADP(+) , but not the 2'-hydroxyl group of NAD(+) , were consistent with a preference for NADP(+) over NAD(+) . The C5-OH and C6-methyl groups of L-rhamnose were recognized by specific residues of RhaDH through hydrogen bonds and hydrophobic contact, respectively, which contribute to the different substrate specificities from other aldose 1-dehydrogenases in the short-chain dehydrogenase/reductase superfamily. | ||
| + | |||
| + | Crystal structure of L-rhamnose 1-dehydrogenase involved in the non-phosphorylative pathway of L-rhamnose metabolism in bacteria.,Yoshiwara K, Watanabe S, Watanabe Y FEBS Lett. 2021 Jan 22. doi: 10.1002/1873-3468.14046. PMID:33482017<ref>PMID:33482017</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7do5" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Azotobacter vinelandii DJ]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Watanabe S]] | [[Category: Watanabe S]] | ||
[[Category: Watanabe Y]] | [[Category: Watanabe Y]] | ||
[[Category: Yoshiwara K]] | [[Category: Yoshiwara K]] | ||
Current revision
Crystal structure of Azotobacter vinelandii L-rhamnose 1-dehydrogenase(apo-form)
| |||||||||||
