6s68
From Proteopedia
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==Structure of the Fluorescent Protein AausFP2 from Aequorea cf. australis at pH 7.6== | ==Structure of the Fluorescent Protein AausFP2 from Aequorea cf. australis at pH 7.6== | ||
| - | <StructureSection load='6s68' size='340' side='right'caption='[[6s68]]' scene=''> | + | <StructureSection load='6s68' size='340' side='right'caption='[[6s68]], [[Resolution|resolution]] 2.06Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S68 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6s68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_australis Aequorea australis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S68 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s68 OCA], [https://pdbe.org/6s68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s68 RCSB], [https://www.ebi.ac.uk/pdbsum/6s68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s68 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.06Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PIA:[(4Z)-2-[(1S)-1-AMINOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>PIA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s68 OCA], [https://pdbe.org/6s68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s68 RCSB], [https://www.ebi.ac.uk/pdbsum/6s68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s68 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Using mRNA sequencing and de novo transcriptome assembly, we identified, cloned, and characterized 9 previously undiscovered fluorescent protein (FP) homologs from Aequorea victoria and a related Aequorea species, with most sequences highly divergent from A. victoria green fluorescent protein (avGFP). Among these FPs are the brightest green fluorescent protein (GFP) homolog yet characterized and a reversibly photochromic FP that responds to UV and blue light. Beyond green emitters, Aequorea species express purple- and blue-pigmented chromoproteins (CPs) with absorbances ranging from green to far-red, including 2 that are photoconvertible. X-ray crystallography revealed that Aequorea CPs contain a chemically novel chromophore with an unexpected crosslink to the main polypeptide chain. Because of the unique attributes of several of these newly discovered FPs, we expect that Aequorea will, once again, give rise to an entirely new generation of useful probes for bioimaging and biosensing. | ||
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| + | Aequorea's secrets revealed: New fluorescent proteins with unique properties for bioimaging and biosensing.,Lambert GG, Depernet H, Gotthard G, Schultz DT, Navizet I, Lambert T, Adams SR, Torreblanca-Zanca A, Chu M, Bindels DS, Levesque V, Nero Moffatt J, Salih A, Royant A, Shaner NC PLoS Biol. 2020 Nov 2;18(11):e3000936. doi: 10.1371/journal.pbio.3000936., eCollection 2020 Nov. PMID:33137097<ref>PMID:33137097</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6s68" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Aequorea australis]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Depernet H]] | [[Category: Depernet H]] | ||
Current revision
Structure of the Fluorescent Protein AausFP2 from Aequorea cf. australis at pH 7.6
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