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Protein Hfq

From Proteopedia

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<StructureSection load='4ht9' size='340' side='right' caption='Structure of ''E. coli'' trimeric Hfq complex with poly(A) RNA (yellow, cyan) and poly(U) RNA (magenta, green) (PDB code [[4ht9]])' scene='87/875687/Cv/1'>
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<StructureSection load='4ht9' size='340' side='right' caption='Structure of E. coli hexameric Hfq complex with poly(A) RNA and poly(U) RNA (PDB code [[4ht9]])' scene='87/875687/Cv/1'>
== Function ==
== Function ==
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== Structural highlights ==
== Structural highlights ==
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The 3D structure of the complex between Hfq and poly(A) RNA and poly(U) RNA shows the poly(A) binding at the distal face of Hfq and poly(U) binding at its proximal face. For the poly(A) A-R-N binding site the A site shows hydrogen bonding to Gln and Lys. The R site shows stacking interactions as well as hydrogen bonding. The N site does not show any interaction. The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions<ref>PMID:23605038</ref>.
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The biological assembly of Protein Hfq from ''E. coli'' is <scene name='87/875687/Cv/2'>homohexamer</scene> (PDB code [[4ht9]]). The 3D structure of the complex between Hfq and <scene name='87/875687/Cv/5'>poly(A) RNA and poly(U) RNA</scene> shows the poly(A) binding at the distal face of Hfq and poly(U) binding at its proximal face. For the poly(A) A-R-N binding site the A site shows <scene name='87/875687/Cv/4'>hydrogen bonding to Gln and Lys</scene>. The <scene name='87/875687/Cv/6'>R site shows stacking interactions as well as hydrogen bonding</scene>. The N site does not show any interaction. <scene name='87/875687/Cv/7'>The poly(U) binding site of Hfq is a classical uridine binding pocket with both stacking and hydrogen bonding interactions</scene><ref>PMID:23605038</ref>.
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</StructureSection>
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== 3D Structures of protein Hfq ==
== 3D Structures of protein Hfq ==
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Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
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[[Protein Hfq 3D structures]]
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{{#tree:id=OrganizedByTopic|openlevels=0|
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*Protein Hfq
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**[[1hk9]], [[2y90]], [[2yht]], [[3qhs]], [[4rcb]], [[4rcc]], [[6bdg]] – EcHfq – ''Escherichia coli'' <br />
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**[[4jli]], [[4jri]], [[4jrk]], [[4juv]] – EcHfq (mutant)<br />
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**[[1u1s]], [[1u1t]], [[4j6y]], [[6xyj]] – PaHfq – ''Pseudomonas aeruginosa''<br />
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**[[3inz]], [[3m4g]], [[4mml]], [[4mmk]], [[5i21]] – PaHfq (mutant)<br />
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**[[2ylb]] – StHfq – ''Salmonella typhimurium''<br />
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**[[6gwk]] – Hfq – ''Caulobacter crescentus''<br />
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**[[4nl2]] – LmHfq – ''Listeria monocytogenes''<br />
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**[[4noy]] – LmHfq (mutant)<br />
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**[[5szd]] – AaHfq – ''Aquifex aeolicus''<br />
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**[[3sb2]] – Hfq - ''Herbaspirillum seropedicae''<br />
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**[[1kq1]] – SaHfq – ''Staphylococcus aureus''<br />
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**[[2qtx]], [[4x9c]] – MjHfq – ''Methanococcus jannaschii''<br />
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**[[3hfn]] – Hfq - ''Anabaena''<br />
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*Protein Hfq complex with nucleotide
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**[[3res]] – EcHfq + ADP<br />
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**[[3qo3]] – EcHfq + ATP<br />
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**[[4pno]] – EcHfq + UMP<br />
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**[[4j6x]] – PaHfq + UTP<br />
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**[[3qui]] – PaHfq + ADPNP<br />
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**[[4j5y]] – PaHfq + ATP<br />
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**[[4j6w]] – PaHfq + CTP<br />
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**[[2ylc]] – StHfq + UMP<br />
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**[[5dy9]] – MjHfq (mutant) + AMP<br />
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**[[4x9d]] – MjHfq + UMP<br />
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*Protein Hfq other complexes
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**[[6qlb]] – EcHfq + calpain <br />
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**[[3vu3]] – EcHfq + catalase <br />
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**[[3gib]], [[4ht8]] – EcHfq + poly(A)<br />
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**[[4ht9]], [[5new]] – EcHfq + poly(A) + poly(U)<br />
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**[[3rer]] – EcHfq + poly(U) + ADP<br />
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**[[4qvc]], [[4qvd]] – EcHfq + RNA<br />
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**[[5uk7]] – EcHfq + DNA<br />
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**[[3ahu]], [[3hsb]] – Hfq + RNA – ''Bacillus subtilis''<br />
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**[[6o1k]], [[6o1l]], [[6o1m]] – PaHfq + Crc + RNA – Cryo EM<br />
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**[[4v2s]] – StHfq + sRNA<br />
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**[[4y91]] – Hfq + poly(U) – ''Thermotoga maritima''<br />
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**[[4nl3]] – LmHfq + poly(U) <br />
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**[[5sze]] – AaHfq + poly(U) <br />
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**[[1kq2]] – SaHfq + RNA <br />
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}}
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== References ==
== References ==
<references/>
<references/>
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</StructureSection>
[[Category:Topic Page]]
[[Category:Topic Page]]

Current revision

Structure of E. coli hexameric Hfq complex with poly(A) RNA and poly(U) RNA (PDB code 4ht9)

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Proteopedia Page Contributors and Editors (what is this?)

Michal Harel, Alexander Berchansky

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