7lq5
From Proteopedia
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- | '''Unreleased structure''' | ||
- | + | ==Cryo-EM structure of OmcZ nanowire from Geobacter sulfurreducens== | |
+ | <StructureSection load='7lq5' size='340' side='right'caption='[[7lq5]], [[Resolution|resolution]] 3.40Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[7lq5]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacter_sulfurreducens_PCA Geobacter sulfurreducens PCA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LQ5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LQ5 FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEC:HEME+C'>HEC</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lq5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lq5 OCA], [https://pdbe.org/7lq5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lq5 RCSB], [https://www.ebi.ac.uk/pdbsum/7lq5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lq5 ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/Q74BG5_GEOSL Q74BG5_GEOSL] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | OmcZ nanowires produced by Geobacter species have high electron conductivity (>30 S cm(-1)). Of 111 cytochromes present in G. sulfurreducens, OmcZ is the only known nanowire-forming cytochrome essential for the formation of high-current-density biofilms that require long-distance (>10 microm) extracellular electron transport. However, the mechanisms underlying OmcZ nanowire assembly and high conductivity are unknown. Here we report a 3.5-A-resolution cryogenic electron microscopy structure for OmcZ nanowires. Our structure reveals linear and closely stacked haems that may account for conductivity. Surface-exposed haems and charge interactions explain how OmcZ nanowires bind to diverse extracellular electron acceptors and how organization of nanowire network re-arranges in different biochemical environments. In vitro studies explain how G. sulfurreducens employ a serine protease to control the assembly of OmcZ monomers into nanowires. We find that both OmcZ and serine protease are widespread in environmentally important bacteria and archaea, thus establishing a prevalence of nanowire biogenesis across diverse species and environments. | ||
- | + | Structure of Geobacter cytochrome OmcZ identifies mechanism of nanowire assembly and conductivity.,Gu Y, Guberman-Pfeffer MJ, Srikanth V, Shen C, Giska F, Gupta K, Londer Y, Samatey FA, Batista VS, Malvankar NS Nat Microbiol. 2023 Feb;8(2):284-298. doi: 10.1038/s41564-022-01315-5. Epub 2023 , Feb 2. PMID:36732469<ref>PMID:36732469</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
+ | <div class="pdbe-citations 7lq5" style="background-color:#fffaf0;"></div> | ||
+ | |||
+ | ==See Also== | ||
+ | *[[Cytochrome C 3D structures|Cytochrome C 3D structures]] | ||
+ | == References == | ||
+ | <references/> | ||
+ | __TOC__ | ||
+ | </StructureSection> | ||
+ | [[Category: Geobacter sulfurreducens PCA]] | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Gu Y]] | ||
+ | [[Category: Malvankar NS]] | ||
+ | [[Category: Samatey FA]] | ||
+ | [[Category: Srikanth V]] |
Current revision
Cryo-EM structure of OmcZ nanowire from Geobacter sulfurreducens
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