7lr3
From Proteopedia
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| - | '''Unreleased structure''' | ||
| - | + | ==Complex of Fab 2/6.14 with domain 3 of P. berghei HAP2== | |
| + | <StructureSection load='7lr3' size='340' side='right'caption='[[7lr3]], [[Resolution|resolution]] 2.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7lr3]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/Plasmodium_berghei Plasmodium berghei]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7LR3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7LR3 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSD:3-SULFINOALANINE'>CSD</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7lr3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7lr3 OCA], [https://pdbe.org/7lr3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7lr3 RCSB], [https://www.ebi.ac.uk/pdbsum/7lr3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7lr3 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/HAP2_PLABA HAP2_PLABA] During fertilization, required on male gametes for their fusion with female gametes, and for subsequent ookinete formation in the host (PubMed:18367645, PubMed:18403203, PubMed:21209845, PubMed:29212032). Thereby, required for mosquito-mediated transmission to other animals (PubMed:18367645, PubMed:18403203, PubMed:29212032). Probably initiates the fusion of gamete cell membranes by inserting part of its extracellular domain into the cell membrane of a female gamete (PubMed:20080406).<ref>PMID:18367645</ref> <ref>PMID:18403203</ref> <ref>PMID:21209845</ref> <ref>PMID:29212032</ref> <ref>PMID:20080406</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | HAP2 is a transmembrane gamete fusogen found in multiple eukaryotic kingdoms and is structurally homologous to viral class II fusogens. Studies in Plasmodium have suggested that HAP2 is an attractive target for vaccines that block transmission of malaria. HAP2 has three extracellular domains, arranged in the order D2, D1, and D3. Here, we report monoclonal antibodies against the D3 fragment of Plasmodium berghei HAP2 and crystal structures of D3 in complex with Fab fragments of two of these antibodies, one of which blocks fertilization of Plasmodium berghei in vitro and transmission of malaria in mosquitoes. We also show how this Fab binds the complete HAP2 ectodomain with electron microscopy. The two antibodies cross-react with HAP2 among multiple plasmodial species. Our characterization of the Plasmodium D3 structure, HAP2 ectodomain architecture, and mechanism of inhibition provide insights for the development of a vaccine to block malaria transmission. | ||
| - | + | Structural basis of malaria transmission blockade by a monoclonal antibody to gamete fusogen HAP2.,Feng J, Dong X, DeCosta A, Su Y, Angrisano F, Sala KA, Blagborough AM, Lu C, Springer TA Elife. 2021 Dec 23;10:e74707. doi: 10.7554/eLife.74707. PMID:34939934<ref>PMID:34939934</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7lr3" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Antibody 3D structures|Antibody 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Mus musculus]] | ||
| + | [[Category: Plasmodium berghei]] | ||
| + | [[Category: Dong XC]] | ||
| + | [[Category: Feng J]] | ||
| + | [[Category: Lu CF]] | ||
| + | [[Category: Springer TA]] | ||
Current revision
Complex of Fab 2/6.14 with domain 3 of P. berghei HAP2
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