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7e5u
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7e5u is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Phm7== | |
| - | + | <StructureSection load='7e5u' size='340' side='right'caption='[[7e5u]], [[Resolution|resolution]] 1.62Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[7e5u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrenochaetopsis_sp. Pyrenochaetopsis sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7E5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7E5U FirstGlance]. <br> | |
| - | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.62Å</td></tr> | |
| - | [[Category: | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7e5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7e5u OCA], [https://pdbe.org/7e5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7e5u RCSB], [https://www.ebi.ac.uk/pdbsum/7e5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7e5u ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PHM7_PYRSX PHM7_PYRSX] Diels-Alderase; part of the gene cluster that mediates the biosynthesis of the trans-fused decalin-containing tetramic acid phomasetin, the stereochemical opposite of the HIV-1 integrase inhibitor equisetin (PubMed:29972614). The PKS module of phm1 together with the enoylreductase phm4 catalyze the formation of the polyketide unit which is then conjugated to L-serine by the condensation domain of the phm1 NRPS module (PubMed:29972614). Activity of the Dieckmann cyclase domain (RED) of phm1 results in release of the Dieckmann product intermediate (PubMed:29972614). The Diels-Alderase phm7 then uses the Dieckmann product of phm1 as substrate and catalyzes the Diels-Alder cycloaddition to form the decalin ring of N-desmethylphomasetin (PubMed:29972614, PubMed:34121297). N-desmethylphomasetin is further methylated to phomasetin by the methyltransferase phm5 (PubMed:29972614).<ref>PMID:29972614</ref> <ref>PMID:34121297</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Pyrenochaetopsis sp]] | ||
| + | [[Category: Fujiyama K]] | ||
| + | [[Category: Hino T]] | ||
| + | [[Category: Kato N]] | ||
| + | [[Category: Kinugasa K]] | ||
| + | [[Category: Nagano S]] | ||
| + | [[Category: Takahashi S]] | ||
Current revision
Crystal structure of Phm7
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