7app
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==Structure of Lipase TL from capillary grown crystal in the presence of agarose== | ==Structure of Lipase TL from capillary grown crystal in the presence of agarose== | ||
| - | <StructureSection load='7app' size='340' side='right'caption='[[7app]]' scene=''> | + | <StructureSection load='7app' size='340' side='right'caption='[[7app]], [[Resolution|resolution]] 1.70Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7APP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7APP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7app]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermomyces_lanuginosus Thermomyces lanuginosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7APP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7APP FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7app FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7app OCA], [https://pdbe.org/7app PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7app RCSB], [https://www.ebi.ac.uk/pdbsum/7app PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7app ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7app FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7app OCA], [https://pdbe.org/7app PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7app RCSB], [https://www.ebi.ac.uk/pdbsum/7app PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7app ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/LIP_THELA LIP_THELA] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The autoimmobilization of enzymes via cross-linked enzyme crystals (CLECs) has regained interest in recent years, boosted by the extensive knowledge gained in protein crystallization, the decrease of cost and laboriousness of the process, and the development of potential applications. In this work, we present the crystallization and preparative-scale production of reinforced cross-linked lipase crystals (RCLLCs) using a commercial detergent additive as a raw material. Bulk crystallization was carried out in 500 mL of agarose media using the batch technique. Agarose facilitates the homogeneous production of crystals, their cross-linking treatment, and their extraction. RCLLCs were active in an aqueous solution and in hexane, as shown by the hydrolysis of p-nitrophenol butyrate and alpha-methylbenzyl acetate, respectively. RCLLCs presented both high thermal and robust operational stability, allowing the preparation of a packed-bed chromatographic column to work in a continuous flow. Finally, we determined the three-dimensional (3D) models of this commercial lipase crystallized with and without phosphate at 2.0 and 1.7 A resolutions, respectively. | ||
| + | |||
| + | Production of Cross-Linked Lipase Crystals at a Preparative Scale.,Fernandez-Penas R, Verdugo-Escamilla C, Martinez-Rodriguez S, Gavira JA Cryst Growth Des. 2021 Mar 3;21(3):1698-1707. doi: 10.1021/acs.cgd.0c01608. Epub , 2021 Feb 17. PMID:34602865<ref>PMID:34602865</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7app" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Lipase 3D Structures|Lipase 3D Structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Thermomyces lanuginosus]] | ||
[[Category: Fernande-Penas R]] | [[Category: Fernande-Penas R]] | ||
[[Category: Gavira JA]] | [[Category: Gavira JA]] | ||
[[Category: Martinez-Rodriguez S]] | [[Category: Martinez-Rodriguez S]] | ||
[[Category: Verdugo-Escamilla C]] | [[Category: Verdugo-Escamilla C]] | ||
Current revision
Structure of Lipase TL from capillary grown crystal in the presence of agarose
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