1du0

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Current revision (06:59, 7 February 2024) (edit) (undo)
 
(12 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1du0.jpg|left|200px]]
 
-
<!--
+
==ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX==
-
The line below this paragraph, containing "STRUCTURE_1du0", creates the "Structure Box" on the page.
+
<StructureSection load='1du0' size='340' side='right'caption='[[1du0]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-
You may change the PDB parameter (which sets the PDB file loaded into the applet)
+
== Structural highlights ==
-
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
+
<table><tr><td colspan='2'>[[1du0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DU0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DU0 FirstGlance]. <br>
-
or leave the SCENE parameter empty for the default display.
+
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-
-->
+
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1du0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1du0 OCA], [https://pdbe.org/1du0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1du0 RCSB], [https://www.ebi.ac.uk/pdbsum/1du0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1du0 ProSAT]</span></td></tr>
-
{{STRUCTURE_1du0| PDB=1du0 | SCENE= }}
+
</table>
-
 
+
== Function ==
-
'''ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX'''
+
[https://www.uniprot.org/uniprot/HMEN_DROME HMEN_DROME] This protein specifies the body segmentation pattern. It is required for the development of the central nervous system. Transcriptional regulator that represses activated promoters. Wg signaling operates by inactivating the SGG repression of EN autoactivation.
-
 
+
== Evolutionary Conservation ==
-
 
+
[[Image:Consurf_key_small.gif|200px|right]]
-
==Overview==
+
Check<jmol>
-
We have determined the crystal structure of a complex containing the engrailed homeodomain Gln50 --&gt; Ala variant (QA50) bound to the wild-type optimal DNA site (TAATTA) at 2.0 A resolution. Biochemical and genetic studies by other groups have suggested that residue 50 is an important determinant of differential DNA-binding specificity among homeodomains (distinguishing among various sites of the general form TAATNN). However, biochemical studies of the QA50 variant had revealed that it binds almost as tightly as the wild-type protein and with only modest changes in specificity. We have now determined the crystal structure of the QA50 variant to help understand the role of residue 50 in site-specific recognition. Our cocrystal structure shows some interesting changes in the water structure at the site of the substitution and shows some changes in the conformations of neighboring side chains. However, the structure, like the QA50 biochemical data, suggests that Gln50 plays a relatively modest role in determining the affinity and specificity of the engrailed homeodomain.
+
<jmolCheckbox>
-
 
+
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/du/1du0_consurf.spt"</scriptWhenChecked>
-
==About this Structure==
+
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
-
1DU0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DU0 OCA].
+
<text>to colour the structure by Evolutionary Conservation</text>
-
 
+
</jmolCheckbox>
-
==Reference==
+
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1du0 ConSurf].
-
Exploring the role of glutamine 50 in the homeodomain-DNA interface: crystal structure of engrailed (Gln50 --&gt; ala) complex at 2.0 A., Grant RA, Rould MA, Klemm JD, Pabo CO, Biochemistry. 2000 Jul 18;39(28):8187-92. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10889025 10889025]
+
<div style="clear:both"></div>
 +
__TOC__
 +
</StructureSection>
[[Category: Drosophila melanogaster]]
[[Category: Drosophila melanogaster]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Grant, R A.]]
+
[[Category: Grant RA]]
-
[[Category: Klemm, J D.]]
+
[[Category: Klemm JD]]
-
[[Category: Pabo, C O.]]
+
[[Category: Pabo CO]]
-
[[Category: Rould, M A.]]
+
[[Category: Rould MA]]
-
[[Category: Dna-binding protein]]
+
-
[[Category: Homeodomain]]
+
-
[[Category: Protein-dna complex]]
+
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 14:16:22 2008''
+

Current revision

ENGRAILED HOMEODOMAIN Q50A VARIANT DNA COMPLEX

PDB ID 1du0

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1du0"
Personal tools