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| | ==SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C== | | ==SOLUTION STRUCTURE OF THE INSECTICIDAL NEUROTOXIN J-ATRACOTOXIN-HV1C== |
| - | <StructureSection load='1dl0' size='340' side='right'caption='[[1dl0]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='1dl0' size='340' side='right'caption='[[1dl0]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1dl0]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DL0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1dl0]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hadronyche_versuta Hadronyche versuta]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1DL0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1DL0 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl0 OCA], [https://pdbe.org/1dl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dl0 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1dl0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1dl0 OCA], [https://pdbe.org/1dl0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1dl0 RCSB], [https://www.ebi.ac.uk/pdbsum/1dl0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1dl0 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/TK1C_HADVE TK1C_HADVE]] This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.<ref>PMID:10881200</ref> <ref>PMID:17141372</ref> <ref>PMID:18625007</ref> <ref>PMID:18986214</ref>
| + | [https://www.uniprot.org/uniprot/TK1C_HADVE TK1C_HADVE] This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.<ref>PMID:10881200</ref> <ref>PMID:17141372</ref> <ref>PMID:18625007</ref> <ref>PMID:18986214</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Hadronyche versuta]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: King, G F]] | + | [[Category: King GF]] |
| - | [[Category: Wang, X H]] | + | [[Category: Wang XH]] |
| - | [[Category: Atracotoxin]]
| + | |
| - | [[Category: Cystine knot]]
| + | |
| - | [[Category: Insecticidal]]
| + | |
| - | [[Category: Neurotoxin]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Vicinal disulfide]]
| + | |
| Structural highlights
Function
TK1C_HADVE This excitatory toxin inhibits insect calcium-activated potassium (KCa) channels (Slo-type). Pan-neuronal expression in Drosophila is lethal but flies engineered to express the toxin only in clock neurons have defects in circadian rhythm but a normal lifespan.[1] [2] [3] [4]
Publication Abstract from PubMed
We have isolated a family of insect-selective neurotoxins from the venom of the Australian funnel-web spider that appear to be good candidates for biopesticide engineering. These peptides, which we have named the Janus-faced atracotoxins (J-ACTXs), each contain 36 or 37 residues, with four disulfide bridges, and they show no homology to any sequences in the protein/DNA databases. The three-dimensional structure of one of these toxins reveals an extremely rare vicinal disulfide bridge that we demonstrate to be critical for insecticidal activity. We propose that J-ACTX comprises an ancestral protein fold that we refer to as the disulfide-directed beta-hairpin.
Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge.,Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:http://dx.doi.org/10.1038/75921
- ↑ Tedford HW, Maggio F, Reenan RA, King G. A model genetic system for testing the in vivo function of peptide toxins. Peptides. 2007 Jan;28(1):51-6. Epub 2006 Dec 1. PMID:17141372 doi:http://dx.doi.org/10.1016/j.peptides.2006.08.026
- ↑ Gunning SJ, Maggio F, Windley MJ, Valenzuela SM, King GF, Nicholson GM. The Janus-faced atracotoxins are specific blockers of invertebrate K(Ca) channels. FEBS J. 2008 Aug;275(16):4045-59. doi: 10.1111/j.1742-4658.2008.06545.x. Epub, 2008 Jul 9. PMID:18625007 doi:http://dx.doi.org/10.1111/j.1742-4658.2008.06545.x
- ↑ Wu Y, Cao G, Pavlicek B, Luo X, Nitabach MN. Phase coupling of a circadian neuropeptide with rest/activity rhythms detected using a membrane-tethered spider toxin. PLoS Biol. 2008 Nov 4;6(11):e273. doi: 10.1371/journal.pbio.0060273. PMID:18986214 doi:http://dx.doi.org/10.1371/journal.pbio.0060273
- ↑ Wang X, Connor M, Smith R, Maciejewski MW, Howden ME, Nicholson GM, Christie MJ, King GF. Discovery and characterization of a family of insecticidal neurotoxins with a rare vicinal disulfide bridge. Nat Struct Biol. 2000 Jun;7(6):505-13. PMID:10881200 doi:http://dx.doi.org/10.1038/75921
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