7dtk
From Proteopedia
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==Crystal structure of the RecA1 domain of RNA helicase CGH-1 in C. elegans== | ==Crystal structure of the RecA1 domain of RNA helicase CGH-1 in C. elegans== | ||
| - | <StructureSection load='7dtk' size='340' side='right'caption='[[7dtk]]' scene=''> | + | <StructureSection load='7dtk' size='340' side='right'caption='[[7dtk]], [[Resolution|resolution]] 1.85Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DTK FirstGlance]. <br> | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7DTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7DTK FirstGlance]. <br> | ||
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dtk OCA], [https://pdbe.org/7dtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dtk RCSB], [https://www.ebi.ac.uk/pdbsum/7dtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dtk ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.849Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7dtk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7dtk OCA], [https://pdbe.org/7dtk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7dtk RCSB], [https://www.ebi.ac.uk/pdbsum/7dtk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7dtk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | A protein-RNA complex containing the RNA helicase CGH-1 and a germline specific RNA-binding protein CAR-1 is involved in various aspects of function in C. elegans. However, the structural basis for the assembly of this protein complex remains unclear. Here, we elucidate the molecular basis of the recognition of CGH-1 by CAR-1. Additionally, we found that the ATPase activity of CGH-1 is stimulated by NTL-1a MIF4G domain in vitro. Furthermore, we determined the structures of the two RecA-like domains of CGH-1 by X-ray crystallography at resolutions of 1.85 and 2.40 A, respectively. Structural and biochemical approaches revealed a bipartite interface between CGH-1 RecA2 and the FDF-TFG motif of CAR-1. NMR and structure-based mutations in CGH-1 RecA2 or CAR-1 attenuated or disrupted CGH-1 binding to CAR-1, assessed by ITC and GST-pulldown in vitro. These findings provide insights into a conserved mechanism in the recognition of CGH-1 by CAR-1. Together, our data provide the missing physical links in understanding the assembly and function of CGH-1 and CAR-1 in C. elegans. | ||
| + | |||
| + | Structural and biochemical insights into the recognition of RNA helicase CGH-1 by CAR-1 in C. elegans.,Zhang Y, Lv M, Li F, Li M, Zhang J, Shi Y, Hong J Biochem Biophys Res Commun. 2021 Mar 3;549:135-142. doi:, 10.1016/j.bbrc.2021.02.119. PMID:33676181<ref>PMID:33676181</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7dtk" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Current revision
Crystal structure of the RecA1 domain of RNA helicase CGH-1 in C. elegans
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