6xo1

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==Crystal structure of N97A mutant of human CEACAM1==
==Crystal structure of N97A mutant of human CEACAM1==
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<StructureSection load='6xo1' size='340' side='right'caption='[[6xo1]]' scene=''>
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<StructureSection load='6xo1' size='340' side='right'caption='[[6xo1]], [[Resolution|resolution]] 1.76&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XO1 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6xo1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XO1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XO1 FirstGlance]. <br>
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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xo1 OCA], [https://pdbe.org/6xo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xo1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xo1 ProSAT]</span></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.758&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xo1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xo1 OCA], [https://pdbe.org/6xo1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xo1 RCSB], [https://www.ebi.ac.uk/pdbsum/6xo1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xo1 ProSAT]</span></td></tr>
</table>
</table>
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== Function ==
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[https://www.uniprot.org/uniprot/CEAM1_HUMAN CEAM1_HUMAN]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Human (h) carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) function depends upon IgV-mediated homodimerization or heterodimerization with host ligands, including hCEACAM5, hTIM-3, PD-1, and a variety of microbial pathogens. However, there is little structural information available on how hCEACAM1 transitions between monomeric and dimeric states which in the latter case is critical for initiating hCEACAM1 activities. We therefore mutated residues within the hCEACAM1 IgV GFCC' face including V39, I91, N97, and E99 and examined hCEACAM1 IgV monomer-homodimer exchange using differential scanning fluorimetry, multi-angle light scattering, X-ray crystallography and/or nuclear magnetic resonance. From these studies, we describe hCEACAM1 homodimeric, monomeric and transition states at atomic resolution and its conformational behavior in solution through NMR assignment of the wildtype (WT) hCEACAM1 IgV dimer and N97A mutant monomer. These studies reveal the flexibility of the GFCC' face and its important role in governing the formation of hCEACAM1 dimers and selective heterodimers.
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Structural basis of the dynamic human CEACAM1 monomer-dimer equilibrium.,Gandhi AK, Sun ZJ, Kim WM, Huang YH, Kondo Y, Bonsor DA, Sundberg EJ, Wagner G, Kuchroo VK, Petsko GA, Blumberg RS Commun Biol. 2021 Mar 19;4(1):360. doi: 10.1038/s42003-021-01871-2. PMID:33742094<ref>PMID:33742094</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6xo1" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Blumberg RS]]
[[Category: Blumberg RS]]

Current revision

Crystal structure of N97A mutant of human CEACAM1

PDB ID 6xo1

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