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7m69
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 7m69 is ON HOLD Authors: Description: Category: Unreleased Structures) |
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| - | '''Unreleased structure''' | ||
| - | + | ==E1435Q Ycf1 mutant in inward-facing wide conformation== | |
| + | <StructureSection load='7m69' size='340' side='right'caption='[[7m69]], [[Resolution|resolution]] 3.42Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[7m69]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7M69 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7M69 FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7m69 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7m69 OCA], [https://pdbe.org/7m69 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7m69 RCSB], [https://www.ebi.ac.uk/pdbsum/7m69 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7m69 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/YCFI_YEAST YCFI_YEAST] Cooperates for the ATP-dependent vacuolar transport of bilirubin and glutathione conjugates.<ref>PMID:10790694</ref> <ref>PMID:8626454</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Yeast Cadmium Factor 1 (Ycf1) sequesters heavy metals and glutathione into the vacuole to counter cell stress. Ycf1 belongs to the ATP binding cassette C-subfamily (ABCC) of transporters, many of which are regulated by phosphorylation on intrinsically-disordered domains. The regulatory mechanism of phosphorylation is still poorly understood. Here, we report two cryo-EM structures of Ycf1 at 3.4 A and 4.0 A resolution in inward-facing open conformations that capture previously unobserved ordered states of the intrinsically disordered regulatory domain (R-domain). R-domain phosphorylation is clearly evident and induces a topology promoting electrostatic and hydrophobic interactions with Nucleotide Binding Domain 1 (NBD1) and the Lasso motif. These interactions stay constant between the structures and are related by rigid body movements of the NBD1/R-domain complex. Biochemical data further show R-domain phosphorylation reorganizes the Ycf1 architecture and is required for maximal ATPase activity. Together, we provide insights into how R-domains control ABCC transporter activity. | ||
| - | + | The structural basis for regulation of the glutathione transporter Ycf1 by regulatory domain phosphorylation.,Khandelwal NK, Millan CR, Zangari SI, Avila S, Williams D, Thaker TM, Tomasiak TM Nat Commun. 2022 Mar 11;13(1):1278. doi: 10.1038/s41467-022-28811-w. PMID:35277487<ref>PMID:35277487</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 7m69" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Saccharomyces cerevisiae S288C]] | ||
| + | [[Category: Khandelwal NK]] | ||
| + | [[Category: Millan CR]] | ||
| + | [[Category: Thaker TM]] | ||
| + | [[Category: Tomasiak TM]] | ||
Current revision
E1435Q Ycf1 mutant in inward-facing wide conformation
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